Energy metabolism of in vitro produced bovine embryos and its relationship to embryo quality.

• Grant number: 12/20535-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: February 01, 2013
• End date: May 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcella Pecora Milazzotto
• Grantee: Carlos Alexandre Soares
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

Currently, several technologies have been explored and have been suggested to evidence metabolic differences between embryos to identifying those most able to generate pregnancy. However, the commercial application of these technologies remains a challenge, mainly because they require very complex equipment and skilled labor, which seems to be financially unfeasible for commercial application. Thus, there remains a need to develop a technology to predict the viability of embryos in a non-invasive, quick and economically feasible to its comercial application. Thus, there remains a need the development of new technologies that allow predicting the viability of the embryos in a non-invasive and rapid way. In vitro production of bovine embryos can present different rates of embryonic development and it is partially related to energy metabolism, which is reflected in the viability of these embryos. Furthermore, by differentially metabolic characteristics, these embryos could also consume and secrete differentially molecules into the medium to serve as biomarkers of embryonic viability. In this sense the evaluation of these molecules could allow the identification of higher quality embryos. This project aims to identify characteristics related to energy metabolism and determine a possible correlation between them and embryo quality. Additionally, we intend to relate these characteristics to the pattern of consumption and production of molecules related to energy metabolism looking for a possible biomarker for embryo viability.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MILAZZOTTO, MARCELLA PECORA; GOISSIS, MARCELO DEMARCHI; CHITWOOD, JAMES LEE; ANNES, KELLY; SOARES, CARLOS ALEXANDRE; ISPADA, JESSICA; ORTIZ AVILA ASSUMPCAO, MAYRA ELENA; ROSS, PABLO JUAN. Early cleavages influence the molecular and the metabolic pattern of individually cultured bovine blastocysts. Molecular Reproduction and Development, v. 83, n. 4, p. 324-336, APR 2016. (13/13199-0, 12/20535-3, 12/10351-2)