Damage in nuclear and Mitochondrial DNA in pregnant women and newborns at different hyperglycemia levels

• Grant number: 12/23296-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): June 01, 2013
• Effective date (End): August 31, 2013
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Iracema de Mattos Paranhos Calderon
• Grantee: Jusciele Brogin Moreli
• Supervisor: Ronald Paul Mason
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Research place: National Institute of Environmental Health Sciences (NIEHS), United States
• Associated to the scholarship: 11/13562-1 - Dna damage and its cellular response in pregnant women and newborns at different hyperglycemia levels, BP.DR

Abstract

Hyperglycemia causes generation of free radicals which leads to oxidative stress and DNA damage. This study will evaluate the damage in nuclear and mitochondrial DNA of pregnant women and infants with different degrees of hyperglycemia. The pregnant women will receive 75g glucose tolerance test (GTT-75g) and glycemic profile (GP) between the 24th and 28th gestational weeks. According to the results, pregnant women will classified in: nondiabetic [ND; normal GTT-75g and GP], mild gestational hyperglycemia [MGH; normal GTT-75g and abnormal GP], gestational diabetes mellitus [GDM; abnormal GTT-75g and GP during pregnancy] and type 2 diabetes [DM2; abnormal GTT-75g before getting pregnant]. Maternal glycemic control will be evaluated by HbA1c levels (HPLC chromatography) and by glycemia mean performed in GP. DNA damages will be evaluated by gene-specified PCR (QPCR). The hypothesis is that hyperglycemia during pregnancy leads to oxidative stress resulting in hight rates of nuclear and mitochondrial DNA damage in both mothers and infants due to the presence of hyperglycemia and hypoxia in the intrauterine environment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORELI, JUSCIELE BROGIN; SANTOS, JANINE HERTZOG; ROCHA, CLARISSA RIBEIRO; DAMASCENO, DEBORA CRISTINA; MORCELI, GLILCIANE; RUDGE, MARILZA VIEIRA; BEVILACQUA, ESTELA; PARANHOS CALDERON, IRACEMA MATTOS. DNA Damage and Its Cellular Response in Mother and Fetus Exposed to Hyperglycemic Environment. BIOMED RESEARCH INTERNATIONAL, 2014. (11/18240-2, 12/23296-0, 11/13562-1)

MORELI, JUSCIELE B.; SANTOS, JANINE H.; LORENZON-OJEA, ALINE RODRIGUES; CORREA-SILVA, SIMONE; FORTUNATO, RODRIGO S.; ROCHA, CLARISSA RIBEIRO; RUDGE, MARILZA V.; DAMASCENO, DEBORA C.; BEVILACQUA, ESTELA; CALDERON, IRACEMA M.. Hyperglycemia Differentially Affects Maternal and Fetal DNA Integrity and DNA Damage Response. International Journal of Biological Sciences, v. 12, n. 4, p. 466-477, 2016. (11/18240-2, 12/23296-0, 11/13562-1)