Analysis of NY-ESO-1 expression in cutaneous melanoma

• Grant number: 12/19168-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: December 01, 2012
• End date: April 30, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Cyro Festa Neto
• Grantee: Mara Huffenbaecher Giavina-Bianchi
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 13/02907-3 - Analysis of NY-ESO-1 expression in cutaneous melanoma, BE.EP.DD

Abstract

Cutaneous melanoma is a major problem worldwide because its incidence and mortality are increasing, meanly in Caucasian individuals. Cancer-testis antigens are characterized by having a low expression in normal tissue and by having an abnormal expression in a variety of cancers. They are a potential target for vaccines against cancer. NY-ESO-1 is the best antigen characterized. It is expressed in 20-30% of all pulmonary, esophagous, liver, stomach, prostate, ovarian, bladder and melanoma cancers. Objectives: to determine the frequency of NY-ESO-1 positive melanomas in our sample and to correlate NY-ESO-1 antigen expression with the clinical and histopathological aspects of cutaneous melanoma. Methods: this will be a retrospective cohort. We will select 90 patients. They will be separated in three groups: 1- Thin melanoma (in situ or thickness < 1.1mm); 2- Intermediate (from 1.1 to 4.0mm); and 3- Thick tumors (> 4.0mm). Their clinical files will be revised and the clinical and histopathological features will be noted. The tumor specimen will be revised and we will perform immunohistochemistry study for NY-ESO-1, CD4+ and CD8+ T cells, Granzyme B and TIA1in the inflammatory infiltrate. We will analyse if there will be statistic difference between positive and negative NY-ESO-1 groups regarding gender, race, skin phototype, age, tumor location, pre-existing lesion, and histopathological type, Breslow index, ulceration, development of metastasis, survival rate and composition of tumor inflammatory infiltrate between these two groups. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GIAVINA-BIANCHI, Mara Huffenbaecher. NY-ESO-1 protein analysis in cutaneous melanoma. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.