Study of migratory, effector and regulatory patterns of cell present in the central nervous system during the inflammatory process of Experimental Autoimmune Encephalomyelitis

• Grant number: 12/21172-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2013
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Alessandro dos Santos Farias
• Grantee: Gleidy Ana Araujo Silva
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 11/18728-5 - Study of migratory, effector and regulatory pattern of autoreactive t lymphocytes, previously transduced with GFP in experimental demyelinating diseases, AP.JP

Abstract

The experimental auto-immune encephalomyelitis (EAE) is an autoimmune T cells mediated diseases, since this disease can be adoptively transferred to naive animals by T CD4 lymphocytes transfer. The effector function of these lymphocytes, however, can be decreased by regulatory T lymphocytes. On the other hand, the auto-reactive T lymphocytes as well as the regulatory T lymphocytes are identified within the T CD4 lymphocytes population. Therefore, the labeling of effectors T lymphocytes opens up a possibility of understanding the migration of these lymphocytes to the nervous system (central or peripheral), as well as the interaction between the auto-reactive and regulatory cells that co-migrate to attenuate the inflammation on the nervous system. In this study, we will use the GFP retroviral transduction technique in auto-reactive T lymphocytes. This method allows a permanent labeling, allowing in vitro, ex vivo and in vivo analysis of auto-reactive T lymphocytes, by cytometry flow and/or confocal microscopy. Besides that, this method of labeling will allow us the track of the auto-reactive T CD4 lymphocytes during its migration as well as cells that comygrate with encephalitogenic t cells into the CNS.