Extracts of the roots of Pfaffia glomerata and Pfaffia paniculata, and fruits of Melia azedarach will be obtained by Soxhlet extraction with polar solvents. Fractionation of these extracts, that are rich in saponins, will be studied by using two different methods of supercritical technology: 1) fractionation of dry extract using a fixed-bed sequential extraction with supercritical carbon dioxide (scCO2), scCO2/ethanol and ethanol/water, and 2) fractionation by precipitation from a hydroalcoholic solution using scCO2 as anti-solvent in a sequence of separation tanks at 50 oC where the pressure will be reduced from 300 bar to 80 bar stage by stage. The hydroalcoholic solution will be produced from the resolubilization of dry extracts in a solution ethanol:water (70:30). All samples obtained from the fractionation will be characterized as yield fractionation and presence of saponins of interest. The fractionation of extracts Pfaffia spp. will be monitored by thin layer chromatography (TLC) using as reference samples of ²-ecdysone and isolated saponins from Pfaffia spp. on CPQBA-UNICAMP. TLC analysis and gas chromatography (GC) will be used to monitor the fractionation of Melia sp..
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