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Elicitation of natural products Biosintesis by endophytic microorganisms interaction

Grant number: 12/21803-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2013
Effective date (End): February 28, 2017
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal Investigator:Mônica Tallarico Pupo
Grantee:Andrés Mauricio Caraballo Rodríguez
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):14/01651-8 - Elicitation of natural products biosynthesis from endophytic microorganisms' interactions, BE.EP.DR

Abstract

Natural products are still an important source for discovering novel bioactive molecules that potentially could be useful for drug design. Endophytes are microorganisms living inside of vegetal tissues considered as prolific natural products. They are in continuous microbial competition and host interaction, which make them very attractive for searching new secondary metabolites. It is necessary to highlight that biologic function for most of the molecules in the real environment is unknown. Endophytic fungi have been widely studied due to their potential for producing several classes of secondary metabolites. Recently, research involving actinobacteria is also increasing because of their high potential for production of bioactive molecules. Sub-culturing of isolated endophytic microorganisms can decrease their ability for producing secondary metabolites, probably due to biosynthetic pathway genes silencing. In order to reactivate these metabolic pathways, co-culturing, in which microorganisms interactions are simulated under laboratory conditions, has been well-succeed for inducing or increasing bioactive compounds production as a result of microbial competition. The goal of this research project is to verify the influence of co-culturing endophytic microorganisms (fungi and actinobacteria) and endophytic microorganisms with pathogenic bacteria in the production of natural products. For this purpose, several culture strategies, chromatographic methods for analysis and separation (including HPLC), spectroscopic (NMR) and spectrometric (ESI-MS, MALDI-TOF and nanoDESI) methods will be used. Analysis of data will generate useful information for the comprehension of microbial interactions and, probably, lead to the discovery of novel bioactive compounds.

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHAGAS, FERNANDA OLIVEIRA; PESSOTTI, RITA DE CASSIA; CARABALLO-RODRIGUEZ, ANDRES MAURICIO; PUPO, MONICA TALLARICO. Chemical signaling involved in plant-microbe interactions. CHEMICAL SOCIETY REVIEWS, v. 47, n. 5, p. 1652-1704, MAR 7 2018. Web of Science Citations: 21.
CARABALLO-RODRIGUEZ, ANDRES M.; MAYOR, CASSIA A.; CHAGAS, FERNANDA O.; PUPO, MONICA T. Amphotericin B as an inducer of griseofulvin-containing guttate in the endophytic fungus Xylaria cubensis FLe9. Chemoecology, v. 27, n. 5, p. 177-185, OCT 2017. Web of Science Citations: 2.
CARABALLO-RODRIGUEZ, ANDRES M.; DORRESTEIN, PIETER C.; PUPO, MONICA T. Molecular inter-kingdom interactions of endophytes isolated from Lychnophora ericoides. SCIENTIFIC REPORTS, v. 7, JUL 14 2017. Web of Science Citations: 7.
CHAGAS, FERNANDA OLIVEIRA; CARABALLO-RODRIGUEZ, ANDRES MAURICIO; DORRESTEIN, PIETER C.; PUPO, MONICA TALLARICO. Expanding the Chemical Repertoire of the Endophyte Streptomyces albospinus RLe7 Reveals Amphotericin B as an Inducer of a Fungal Phenotype. Journal of Natural Products, v. 80, n. 5, p. 1302-1309, MAY 2017. Web of Science Citations: 9.
GARG, NEHA; LUZZATTO-KNAAN, TAL; MELNIK, ALEXEY V.; CARABALLO-RODRIGUEZ, ANDRES MAURICIO; FLOROS, DIMITRIOS J.; PETRAS, DANIEL; GREGOR, RACHEL; DORRESTEIN, PIETER C.; PHELAN, VANESSA V. Natural products as mediators of disease. NATURAL PRODUCT REPORTS, v. 34, n. 2, p. 194-219, FEB 1 2017. Web of Science Citations: 22.
CONTI, RAPHAEL; CHAGAS, FERNANDA OLIVEIRA; CARABALLO-RODRIGUEZ, ANDRES MAURICIO; DA PAIXAO MELO, WEILAN GOMES; DO NASCIMENTO, ANDREA MENDES; CAVALCANTI, BRUNO COELHO; DE MORAES, MANOEL ODORICO; PESSOA, CLAUDIA; COSTA-LOTUFO, LETICIA VERAS; KROGH, RENATA; ANDRICOPULO, ADRIANO DEFINI; LOPES, NORBERTO PEPORINE; PUPO, MONICA TALLARICO. Endophytic Actinobacteria from the Brazilian Medicinal Plant Lychnophora ericoides Mart. and the Biological Potential of Their Secondary Metabolites. CHEMISTRY & BIODIVERSITY, v. 13, n. 6, p. 727-736, JUN 2016. Web of Science Citations: 12.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
RODRÍGUEZ, Andrés Mauricio Caraballo. Elicitation of natural products biosynthesis from endophytic microorganisms´ interactions. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.