Copper deficiency and toxicity in Citrus: integrating nutrient absorption and plant nutritional status

• Grant number: 12/13917-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): January 01, 2013
• Effective date (End): November 30, 2016
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Dirceu de Mattos Junior
• Grantee: Franz Walter Rieger Hippler
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated scholarship(s): 15/00720-9 - How copper stress affects nitrogen uptake by Arabidopsis, BE.EP.DR

Abstract

Copper deficiency (Cu) has been observed in non-bearing citrus orchards established on low fertility soils and with reduced foliar applications of fungicides. In either no-bearing or bearing orchard trees, the opposite can occur, caused by the toxicity of the element due to excess applications of fungicides to control foliar and fruit diseases such as citrus canker. In this condition, the concentration of Cu in soil can increase and cause damage to the roots, which severity depends on the rootstock variety. It is noteworthy that the Cu demand of citrus is associated to nitrogen (N), being more pronounced with higher N fertilization. Moreover, the supply of calcium (Ca) can minimize effects of excess Cu in the plant. The objectives are to evaluate the absorption of Cu and the response of orange trees on three rootstocks in conditions of low, adequate and excessive metal availability in nutrient solution; study the interaction between Cu absorption and biochemical parameters with nutritional status of citrus based on the supply of N and Ca; evaluate the effects of foliar versus root uptake of Cu under appropriate conditions defined in the second objective; and study the redistribution of Cu from old tissues to new parts of the orange trees. Four studies interrelated will be conducted. The first (I) will evaluate the absorption and redistribution of Cu in orange trees on different rootstocks (Rangpur lime, Swingle citrumelo and Sunkimandarin) in response to the availability of Cu in nutrient solution (0, 2.0, 20.0 and 200.0 mmol/L). From this study, it will be selected a rootstock combination of interest for the development of other experiments. Experiments II and III will evaluate the absorption of Cu (0, 0.5, 2.0 and 8.0 g/plant) supplied via root and the interaction with N (20 and 60 g/plant) and Ca (0.5 and 30 g/plant) to the soil, respectively. The last experiment (IV) will be held concurrently with the previous two, first combining sources of the element [CuSO4 and Cu(OH)2] and the supply of foliar Cu (0, 0.5, 1.0 and 2.0 g/plant). Comparison of foliar and root absorption by plants will be conducted for selected treatments of experiments II and III with those of IV treatments receiving the same rates of Cu (0.5 to 2.0 g/plant). In all experiments, the effects of treatments will be evaluated based on plant growth, nutritional status and changes in the activity of enzymes related to the metabolism of Cu and stress caused by the deficiency or toxicity of the element. The research results will contribute to a better understanding of nutritional disorders caused by Cu in citrus plants, and consequently create subsidies to optimize production where Cu is a limiting factor, and consequently provide increase in agricultural efficiency and cost in citrus production. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HIPPLER, FRANZ W. R.; DOVIS, VERONICA L.; BOARETTO, RODRIGO M.; QUAGGIO, JOSE A.; AZEVEDO, RICARDO A.; WILLIAMS, LORRAINE E.; MATTOS-, JR., DIRCEU. Photosynthesis is differently regulated during and after copper-induced nutritional stress in citrus trees. PHYSIOLOGIA PLANTARUM, v. 163, n. 3, SI, p. 399-413, JUL 2018. (12/13917-7, 15/13572-8)

HIPPLER, FRANZ W. R.; CIPRIANO, DANILO O.; BOARETTO, RODRIGO M.; QUAGGIO, JOSE A.; GAZIOLA, SALETE A.; AZEVEDO, RICARDO A.; MATTOS-, JR., DIRCEU. Citrus rootstocks regulate the nutritional status and antioxidant system of trees under copper stress. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 130, p. 42-52, OCT 2016. (12/21020-7, 12/13917-7)

HIPPLER, FRANZ W. R.; MATTOS-JR, DIRCEU; BOARETTO, RODRIGO M.; WILLIAMS, LORRAINE E.. Copper excess reduces nitrate uptake by Arabidopsis roots with specific effects on gene expression. Journal of Plant Physiology, v. 228, p. 158-165, SEP 2018. (12/13917-7, 15/00720-9)

MATTOS-JR, D.; HUBER, L. N.; PETENA, G.; BORTOLOTI, G. A.; HIPPLER, F. W. R.; BOARETTO, R. M.. Biochemical and anatomical aspects of copper deficiency induced by high nitrogen supply in Citrus. PLANT AND SOIL, v. N/A, p. 12-pg., 2023-02-06. (12/13917-7)

RIEGER HIPPLER, FRANZ WALTER; BOARETTO, RODRIGO MARCELLI; DOVIS, VERONICA LORENA; QUAGGIO, JOSE ANTONIO; AZEVEDO, RICARDO ANTUNES; MATTOS-, JR., DIRCEU. Oxidative stress induced by Cu nutritional disorders in Citrus depends on nitrogen and calcium availability. SCIENTIFIC REPORTS, v. 8, JAN 26 2018. (12/13917-7, 15/13572-8)

HIPPLER, FRANZ W. R.; DOVIS, VERONICA L.; BOARETTO, RODRIGO M.; QUAGGIO, JOSE A.; AZEVEDO, RICARDO A.; WILLIAMS, LORRAINE E.; MATTOS-, DIRCEU, JR.. Photosynthesis is differently regulated during and after copper-induced nutritional stress in citrus trees. PHYSIOLOGIA PLANTARUM, v. 163, n. 3, p. 15-pg., 2018-07-01. (12/13917-7, 15/13572-8)

RIEGER HIPPLER, FRANZ WALTER; PETENA, GUILHERME; BOARETTO, RODRIGO MARCELLI; QUAGGIO, JOSE ANTONIO; AZEVEDO, RICARDO ANTUNES; MATTOS-, JR., DIRCEU. Mechanisms of copper stress alleviation in Citrus trees after metal uptake by leaves or roots. Environmental Science and Pollution Research, v. 25, n. 13, SI, p. 13134-13146, MAY 2018. (12/13917-7, 15/13572-8)

FERNANDO TREVIZAN DEVITE; FERNANDO ALVES DE AZEVEDO; MARINÊS BASTIANEL; PATRÍCIA MARLUCI DA CONCEIÇÃO; RODRIGO MARCELLI BOARETTO; DIRCEU DE MATTOS JÚNIOR. Respostas de plantas de tangor Murcott IAC 221, supridas com cálcio,à infecção por Alternaria alternata. Revista Brasileira de Fruticultura, v. 45, 2023-06-02. (12/13917-7)

DEVITE, FERNANDO TREVIZAN; DE AZEVEDO, FERNANDO ALVES; BASTIANEL, MARINES; DA CONCEICAO, PATRICIA MARLUCI; BOARETTO, RODRIGO MARCELLI; DE MATTOS JUNIOR, DIRCEU. Responses of calcium-supplied Murcott IAC 221 tangor plants to Alternaria alternata infection. Revista Brasileira de Fruticultura, v. 45, n. 1, p. 12-pg., 2023-01-01. (12/13917-7)

RIEGER HIPPLER, FRANZ WALTER; PETENA, GUILHERME; BOARETTO, RODRIGO MARCELLI; QUAGGIO, JOSE ANTONIO; AZEVEDO, RICARDO ANTUNES; MATTOS-, DIRCEU, JR.. Mechanisms of copper stress alleviation in Citrus trees after metal uptake by leaves or roots. Environmental Science and Pollution Research, v. 25, n. 13, p. 13-pg., 2018-05-01. (12/13917-7, 15/13572-8)