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Rooting of Eucalyptus grandis x Eucalyptus urophylla through the action of hydrogen peroxide and quercetin

Grant number: 12/14886-8
Support type:Scholarships in Brazil - Master
Effective date (Start): January 01, 2013
Effective date (End): February 28, 2014
Field of knowledge:Biological Sciences - Botany - Pant Physiology
Principal Investigator:Giuseppina Pace Pereira Lima
Grantee:Débora Zanoni Do Prado
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated scholarship(s):13/04678-1 - Determination of enzymes polyamine oxidase and peroxidase activity in Eucalyptus grandis x Eucalyptus urophylla, BE.EP.MS


Vegetative propagation is the main form of Eucalyptus seedlings production due to several advantages, but some clonal materials still have difficulties in propagation, including on rooting. Indirectly, flavonoids and hydrogen peroxide can improve rooting by increasing the activity of endogenous auxin. Our aim is to evaluate the action of the flavonoid quercetin and hydrogen peroxide on rooting of Eucalyptus grandis x Eucalyptus urophylla. We will pick up cuttings in clonal garden with hydroponics system developed in conduits. We will develop experiments in a completely randomized design with 8 treatments and 5 replicates of 48 seedlings per experimental unit. We will evaluate the 28 central plants periodically, according to the following criteria: height, diameter and survival rate. After 90 days, we will execute in the 20 central seedlings a destructive evaluation that will determine the percentage of rooting and callus induction, average length of longest root and number of roots. Following, we will combine the best treatments to verify the interaction of hydrogen peroxide and quercetin. We will determine the activity of enzymes IAA oxidase, peroxidase, polyphenoloxidase, phenylalanine ammonia-lyase and quantify the levels of flavonoids, phenols and polyamines (biochemical markers of rooting) in leaves and roots. In the best results, we will, through the technique of liquid chromatography (HPLC), quantify auxin (IAA) and phenolic compounds. We will subject the periodically measured data to analysis of variance for repeated measures and the others to analysis of variance (ANOVA) and means compared by Tukey test at 5% probability.Keywords: polyamines, enzymes, phenolic compounds, biochemical markers.