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GENETIC STRUCTURE OF CACTACEAE SPECIES OF PILOSOCEREUS AURISETUS GROUP USING MICROSATELLITES MARKERS DEVELOPED BY NEXT GENERATION SEQUENCING

Grant number: 12/22857-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2013
Effective date (End): June 30, 2017
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal researcher:Evandro Marsola de Moraes
Grantee:Isabel Aparecida da Silva Bonatelli
Home Institution: Centro de Ciências Humanas e Biológicas (CCHB). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil

Abstract

The group of cacti PILOSOCEREUS AURISETUS is composed of eight species occurring associated with rock outcrops in xeric environments in Northeast, Midwest and Southeast Brazil. The disjunct distribution pattern of populations, along with genetic data suggests that the evolution of this group was influenced by vicariant events involving changes in species distribution and secondary contact with gene flow. Current levels of gene flow are very low and in general the genetic groups are better explained by the geographic distribution of populations than by the current taxonomy. In the Midwest, the genetic structure of P. machrisii, the most widely distributed species, was very complex and can be related to the great morphologic diversity of this region. However the low variability in the markers used and the low sampling in this region did not allow a further understanding of this structure. The aim of this project is to use genomic data to be obtained by next generation sequencing to develop a large set of highly variable microsatellite markers and with uniformity in their mode of evolution. These markers will be used along with an extensive sampling in the Midwest to investigate the history of P. AURISETUS group diversification.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREZ, MANOLO F.; FRANCO, FERNANDO F.; BOMBONATO, JULIANA R.; BONATELLI, ISABEL A. S.; KHAN, GULZAR; ROMEIRO-BRITO, MONIQUE; FEGIES, ANA C.; RIBEIRO, PAULIANNY M.; SILVA, GISLAINE A. R.; MORAES, EVANDRO M. Assessing population structure in the face of isolation by distance: Are we neglecting the problem?. DIVERSITY AND DISTRIBUTIONS, v. 24, n. 12, p. 1883-1889, DEC 2018. Web of Science Citations: 5.
BONATELLI, ISABEL A. S.; CARSTENS, BRYAN C.; MORAES, EVANDRO M. Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae). PLoS One, v. 10, n. 11 NOV 11 2015. Web of Science Citations: 6.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.