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Internal standard addition method using flowing gradient concentration in spectroscopic techniques

Grant number: 12/23323-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2013
Effective date (End): February 28, 2017
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:José Anchieta Gomes Neto
Grantee:Felipe Manfroi Fortunato
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


A new calibration method in analytical chemistry combining the standard addition and internal standardization methods is proposed in this Doctoral Thesis. The internal standard addition using gradient flow analysis is a novel method and benefits in the field of quantitative chemical analysis are: a) correct for matrix or transport effects, and fluctuations due to changes in sample size, instrumental parameters; b) minimal manipulation of sample and standard solutions, c) reduction of reagent consumption and analysis time; d) minimization of contamination and loss of analyte by employing dynamical systems in pipelines; e) analyst exposure (or contact) to numerous chemicals is reduced. The benefits of this proposal are clear and will be demonstrated by using two spectroscopic techniques: high-resolution continuum source flame atomic absorption spectrometry (in the determination of Ca, K, Mg and Na in biodiesel and urine) and Raman spectroscopy (in the determination of uric acid, creatinine and urea in urine; benzene, toluene, ethylbenzene and xylenes - BTEX - in gasoline, methanol in gasoline, ethanol and biodiesel, biodiesel in diesel).

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FORTUNATO, FELIPE MANFROI; BECHLIN, MARCOS ANDRE; FERREIRA, EDILENE CRISTINA; OLIVEIRA, SILVANA RUELLA; GOMES NETO, JOSE ANCHIETA. Evaluation of Nitrate as Internal Standard for Quantitative Determination of Urea in Urine by Raman Spectroscopy. BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY, v. 5, n. 19, p. 22-28, APR-JUN 2018. Web of Science Citations: 0.
FORTUNATO, FELIPE M.; VIEIRA, ALAN L.; GOMES NETO, JOSE A.; DONATI, GEORGE L.; JONES, BRADLEY T. Expanding the potentialities of standard dilution analysis: Determination of ethanol in gasoline by Raman spectroscopy. Microchemical Journal, v. 133, p. 76-80, JUL 2017. Web of Science Citations: 11.
MIRANDA, KELBER; VIEIRA, ALAN LIMA; BECHLIN, MARCOS ANDRE; FORTUNATO, FELIPE MANFROI; VIRGILIO, ALEX; FERREIRA, EDILENE CRISTINA; GOMES NETO, JOSE ANCHIETA. Determination of Ca, Cd, Cu, Fe, K, Mg, Mn, Mo, Na, Se, and Zn in Foodstuffs by Atomic Spectrometry After Sample Preparation Using a Low-Cost Closed-Vessel Conductively Heated Digestion System. FOOD ANALYTICAL METHODS, v. 9, n. 7, p. 1887-1894, JUL 2016. Web of Science Citations: 4.
FORTUNATO, FELIPE M.; BECHLIN, MARCOS A.; GOMES NETO, JOSE A.; VIRGILIO, ALEX; DONATI, GEORGE L.; JONES, BRADLEY T. Standard dilution analysis in flow system: Sodium determination by flame atomic emission spectrometry. Microchemical Journal, v. 124, p. 662-667, JAN 2016. Web of Science Citations: 4.
FORTUNATO, FELIPE MANFROI; BECHLIN, MARCOS A.; GOMES NETO, JOSE A.; DONATI, GEORGE L.; JONES, BRADLEY T. Internal standard addition calibration: Determination of calcium and magnesium by atomic absorption spectrometry. Microchemical Journal, v. 122, p. 63-69, SEP 2015. Web of Science Citations: 9.
BECHLIN, MARCOS ANDRE; FORTUNATO, FELIPE MANFROI; DA SILVA, RICARDO MOUTINHO; FERREIRA, EDILENE CRISTINA; GOMES NETO, JOSE ANCHIETA. A simple and fast method for assessment of the nitrogen-phosphorus-potassium rating of fertilizers using high-resolution continuum source atomic and molecular absorption spectrometry. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, v. 101, p. 240-244, NOV 1 2014. Web of Science Citations: 7.
BECHLIN, MARCOS A.; FORTUNATO, FELIPE M.; FERREIRA, EDILENE C.; GOMES NETO, JOSE A.; NOBREGA, JOAQUIM A.; DONATI, GEORGE L.; JONES, BRADLEY T. Bismuth as a general internal standard for lead in atomic absorption spectrometry. Analytica Chimica Acta, v. 831, p. 24-30, JUN 11 2014. Web of Science Citations: 10.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FORTUNATO, Felipe Manfroi. Avaliação do método de adição de padrão interno em técnicas espectroscópicas. 2017. 164 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Química..

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