Reticulum stress involvement in fatty acids-induced neutrophils death

• Grant number: 12/15079-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2013
• Effective date (End): December 31, 2014
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Adriana Cristina Levada-Pires
• Grantee: Raquel Freitas Zambonatto
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil
• Associated research grant: 10/50848-8 - Role of oxygen reactive species and ceramides in human neutrophil death induced by fatty acids, AP.JP

Abstract

The mechanisms involved in neutrophil apoptosis induced by fatty acids and the involvement of reticulum stress in this process will be investigated. Will be investigated the effect of monounsaturated fatty acid oleic (the main component of olive oil)and the polyunsaturated fatty acid n-6 linoleic (the main component of soybean oil) which is very abundant in the diet. Human neutrophils will be pre-treated for 3 hours with a concentration, non-toxic (100 µM) and toxic (250 µM) of oleic and linoleic acids. The mechanisms involved in AG-induced neutrophil death will be assessed by fluorometry and western blotting. The effect of fatty acids on the expression of pro-(Bax) and antiapoptotic (Mcl-1)proteins. The extrinsic (Bid) and intrinsic pathways (cytochrome c) and/or endoplasmic reticulum stress (CHOP, GRP78, PERK, pPERK, peIF2-alpha, and caspase 12) involved in neutrophils death also will be determined. To establish the possible involvement of reticulum stress in the death of neutrophils, cells are cultivated with the fatty phenyl butyric acid (4-PBA - inhibitor reticulum stress), and then will be determined in neutrophils the viability and DNA fragmentation by the cytometric flow.(AU)