| Grant number: | 12/12190-6 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Effective date (Start): | April 01, 2013 |
| Effective date (End): | January 31, 2014 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Reproduction |
| Principal Investigator: | José Antonio Dell'Aqua Junior |
| Grantee: | Caroline Scott |
| Host Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
Abstract The sexing of sperm, to obtain doses of semen with a high percentage of gametes carrying the X or Y chromosome, even more relevant the role of artificial insemination in maximizing the genetic progress between generations, productivity and economic returns in some farming activities; in assisted reproduction in mammals, and in genetic counseling of the human species. The objectives of this project will remove the plasma membrane proteins X and Y sperm of cattle; analyze the protein profile electrophoresis for uni-and bi-dimensional in order to select the sex-specific proteins, and sequence the selected proteins. Doses will be used for commercial sexed semen (Sexing Technologies, CRV Lagoa da Serra) cytometric sperm selected for X and Y. From these samples membrane proteins are removed by sonication. We will then analyzes the protein profile of the extract of the membrane by electrophoresis uni-and bi-dimensional. After completion of the gels the proteins identified as potential candidates for sex-specific will be transferred to a PVDF membrane and sent to the completion of sequencing. (AU) | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
| TITULO | |
| Articles published in other media outlets (0 total): | |
| More itemsLess items | |
| VEICULO: TITULO (DATA) | |
| VEICULO: TITULO (DATA) | |