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Identification and sequencing of sex-specific protein of bovine sperm

Grant number: 12/12190-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): April 01, 2013
Effective date (End): January 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:José Antonio Dell'Aqua Junior
Grantee:Caroline Scott
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The sexing of sperm, to obtain doses of semen with a high percentage of gametes carrying the X or Y chromosome, even more relevant the role of artificial insemination in maximizing the genetic progress between generations, productivity and economic returns in some farming activities; in assisted reproduction in mammals, and in genetic counseling of the human species. The objectives of this project will remove the plasma membrane proteins X and Y sperm of cattle; analyze the protein profile electrophoresis for uni-and bi-dimensional in order to select the sex-specific proteins, and sequence the selected proteins. Doses will be used for commercial sexed semen (Sexing Technologies, CRV Lagoa da Serra) cytometric sperm selected for X and Y. From these samples membrane proteins are removed by sonication. We will then analyzes the protein profile of the extract of the membrane by electrophoresis uni-and bi-dimensional. After completion of the gels the proteins identified as potential candidates for sex-specific will be transferred to a PVDF membrane and sent to the completion of sequencing. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SCOTT, Caroline. Efeitos da sexagem por citometria de fluxo sobre a composição protéica da membrana de espermatozóides bovinos. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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