INVOLVEMENT OF NITRIC OXIDE ON PHYSIOLOGICAL RESPONSES IN PLANTS OF SUGARCANE UNDER WATER DEFICIT

• Grant number: 12/19167-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): April 01, 2013
• Effective date (End): November 30, 2016
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Rafael Vasconcelos Ribeiro
• Grantee: Neidiquele Maria Silveira
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated research grant: 08/57495-3 - Integrating physiological, morphological and anatomical traits to understand the differential sucrose yield in sugarcane genotypes, AP.BIOEN.TEM
• Associated scholarship(s): 15/21546-7 - Nitric oxide and drought tolerance of sugarcane plants, BE.EP.DR

Abstract

Drought is a major limiting factor in crop yield, plants generally show a reduction in the rates of photosynthesis and growth, often associated with changes in plant metabolism. Nitric oxide (NO) has emerged as an important signaling molecule in various biological systems. It is postulated that NO plays a protective role by acting mainly on the interruption of free radicals formed under oxidative stress. Given the above, our objective is to evaluate the influence of NO in physiological responses of sugar cane (Saccharum spp) to drought stress. Additionally, we intend to determine the main sources of NO in genotypes with differential sensitivity to drought. Genotypes will be subjected to drought by adding PEG 8000 to the nutritive solution (osmotic potential of -0.75 MPa). In addition to evaluating the differential production of NO by plants limiting conditions, we also analyze the role of NO delivered through nutrient solutions of sodium nitroprusside (SNP). Evaluations will be divided into three experiments; the first will deal with the activity of enzymes synthesis, beyond the detection of NO in plants. The second will address the physiological analyzes such as gas exchange, photochemical activity, photosynthetic pigments, enzymes of photosynthetic metabolism and growth analysis in order to verify if NO is capable of attenuating growth inhibition and impairment of the electron transport chain in photosynthesis under stress conditions. The last experiment answer is NO potentiate antioxidant mechanisms, therefore, we will perform analyzes of reactive oxygen species and antioxidant enzyme system. The experiments are conducted in a randomized block design with five replicates and the data subjected to analysis of variance and the means were compared by Tukey test.

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVEIRA, NEIDIQUELE M.; HANCOCK, JOHN T.; FRUNGILLO, LUCAS; SIASOU, ELENI; MARCOS, FERNANDA C. C.; SALGADO, IONE; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane. Plant Physiology and Biochemistry, v. 115, p. 354-359, JUN 2017. (15/21546-7, 12/19167-0)

SILVEIRA, NEIDIQUELE M.; FRUNGILLO, LUCAS; MARCOS, FERNANDA C. C.; PELEGRINO, MILENA T.; MIRANDA, MARCELA T.; SEABRA, AMEDEA B.; SALGADO, IONE; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Exogenous nitric oxide improves sugarcane growth and photosynthesis under water deficit. PLANTA, v. 244, n. 1, p. 181-190, JUL 2016. (12/19167-0, 15/00393-8)

SILVEIRA, NEIDIQUELE M.; SEABRA, AMEDEA B.; MARCOS, FERNANDA C. C.; PELEGRINO, MILENA T.; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Encapsulation of S-nitrosoglutathione into chitosan nanoparticles improves drought tolerance of sugarcane plants. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 84, p. 38-44, MAR 1 2019. (16/10347-6, 18/08194-2, 12/19167-0, 15/00393-8)

MOURA, BARBARA B.; SILVEIRA, NEIDIQUELE M.; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Effects of storage time and freeze-drying on the activity of antioxidant enzymes in sugarcane leaves. Rev. bras. Bot., v. 39, n. 1, p. 4-pg., 2016-03-01. (14/13524-0, 12/19167-0)

SEABRA, AMEDEA B.; SILVEIRA, NEIDIQUELE M.; RIBEIRO, RAFAEL, V; PIERETTI, JOANA C.; BARROSO, JUAN B.; CORPAS, FRANCISCO J.; PALMA, JOSE M.; HANCOCK, JOHN T.; PETRIVALSKY, MAREK; GUPTA, KAPUGANTI J.; et al. Nitric oxide-releasing nanomaterials: from basic research to potential biotechnological applications in agriculture. NEW PHYTOLOGIST, v. 234, n. 4, p. 7-pg., 2022-03-29. (20/03646-2, 12/19167-0, 17/11279-7, 18/08194-2, 19/15095-3, 15/21546-7)

MOURA, BARBARA B.; SILVEIRA, NEIDIQUELE M.; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Effects of storage time and freeze-drying on the activity of antioxidant enzymes in sugarcane leaves. BRAZILIAN JOURNAL OF BOTANY, v. 39, n. 1, p. 373-376, MAR 2016. (14/13524-0, 12/19167-0)

SILVEIRA, NEIDIQUELE M.; MARCOS, FERNANDA C. C.; FRUNGILLO, LUCAS; MOURA, BARBARA B.; SEABRA, AMEDEA B.; SALGADO, IONE; MACHADO, EDUARDO C.; HANCOCK, JOHN T.; RIBEIRO, RAFAEL V.. S-nitrosoglutathione spraying improves stomatal conductance, Rubisco activity and antioxidant defense in both leaves and roots of sugarcane plants under water deficit. PHYSIOLOGIA PLANTARUM, v. 160, n. 4, p. 383-395, AUG 2017. (12/19167-0, 14/13524-0)

MARCOS, FERNANDA C. C.; SILVEIRA, NEIDIQUELE M.; MARCHIORI, PAULO E. R.; MACHADO, EDUARDO C.; SOUZA, GUSTAVO M.; LANDELL, MARCOS G. A.; RIBEIRO, RAFAEL V.. Drought tolerance of sugarcane propagules is improved when origin material faces water deficit. PLoS One, v. 13, n. 12, DEC 26 2018. (08/57495-3, 12/19167-0)

ROBERTO, GUILHERME GARCIA; CUNHA, CAMILA; GABRIEL SALES, CRISTINA RODRIGUES; SILVEIRA, NEIDIQUELE MARIA; RIBEIRO, RAFAEL VASCONCELOS; MACHADO, EDUARDO CARUSO; MAGALHAES ANDRADE LAGOA, ANA MARIA. Variação da fotossíntese e dos teores de carboidratos induzidos por etefom e déficit hídrico na fase de maturação da cana-de-açúcar. Bragantia, v. 74, n. 4, p. 379-386, OCT-DEC 2015. (08/57495-3, 12/13920-8, 12/19167-0)

SILVA, KARINA I.; SALES, CRISTINA R. G.; MARCHIORI, PAULO E. R.; SILVEIRA, NEIDIQUELE M.; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Short-term physiological changes in roots and leaves of sugarcane varieties exposed to H2O2 in root medium. Journal of Plant Physiology, v. 177, p. 93-99, APR 1 2015. (12/19167-0)