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Paratransgenesis in Culex quinquefasciatus mosquitoes

Grant number: 12/19117-2
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): May 01, 2013
Effective date (End): April 30, 2016
Field of knowledge:Health Sciences - Collective Health
Principal Investigator:Mauro Toledo Marrelli
Grantee:André Barretto Bruno Wilke
Home Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Mosquitoes of the Culex genus have great epidemiological importance, are virtually nationwide and have the ability to survive in highly polluted waters reaching very high densities. Traditional control strategies have become ineffective and with high environmental impact.Considerable efforts have been devoted successfully to genetic transformation of various species of mosquitoes such as: Aedes aegypti, Aedes albopictus, Aedes fluviatilis, Anopheles gambiae, Anopheles stephensi. Mosquitoes of the Culex genus, however, have been transformed only once, a decade ago, despite the efforts of several laboratories. Due to this inherent difficulty other forms of control should be considered, such as Paratransgenesis. In this method, a symbiotic bacteria naturally present in mosquitoes might be used as vectors for gene introduction, obtaining similar results as mosquitoes transformation, but with no additional cost fitness.The bacterial design to be utilized in Paratransgenesis requires three key components: (1) effector molecule, (2) excretory mechanism (3) bacteria competency to colonize the mosquito. It is desirable that the bacteria used are capable of colonizing a variety of mosquito species, allowing its use in different isolates and species.By this approach, symbiotic bacteria would be transformed to produce effector molecules and then, reintroduced into the mosquito causing the intended effect.For this strategy to succeed, it needs a better understanding and comprehension of the community microbial structure in the gut of mosquitoes.This study has the following objectives: (1) Identification of bacteria present in Culex quinquefasciatus mosquitoes (2) Bacteria ability to be reintroduced into the mosquito, (3) Identification of effector molecules for use in Culex quinquefasciatus and (4) Potential bacteria to Paratransgenesis. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRUNO WILKE, ANDRE BARRETTO; WILK-DA-SILVA, RAMON; MARRELLI, MAURO TOLEDO. Microgeographic population structuring of Aedes aegypti (Diptera: Culicidae). PLoS One, v. 12, n. 9 SEP 20 2017. Web of Science Citations: 9.
CHRISTE, RAFAEL DE OLIVEIRA; BRUNO WILKE, ANDRE BARRETTO; VIDAL, PALOMA OLIVEIRA; MARRELLI, MAURO TOLEDO. Wing sexual dimorphism in Aedes fluviatilis (Diptera: Culicidae). INFECTION GENETICS AND EVOLUTION, v. 45, p. 434-436, NOV 2016. Web of Science Citations: 3.
MULTINI, LAURA CRISTINA; BRUNO WILKE, ANDRE BARRETTO; SUESDEK, LINCOLN; MARRELLI, MAURO TOLEDO. Population Genetic Structure of Aedes fluviatilis (Diptera: Culicidae). PLoS One, v. 11, n. 9 SEP 6 2016. Web of Science Citations: 9.
BRUNO WILKE, ANDRE BARRETTO; CHRISTE, RAFAEL DE OLIVEIRA; MULTINI, LAURA CRISTINA; VIDAL, PALOMA OLIVEIRA; WILK-DA-SILVA, RAMON; DE CARVALHO, GABRIELA CRISTINA; MARRELLI, MAURO TOLEDO. Morphometric Wing Characters as a Tool for Mosquito Identification. PLoS One, v. 11, n. 8 AUG 23 2016. Web of Science Citations: 11.
MULTINI, LAURA CRISTINA; MARRELLI, MAURO TOLEDO; BRUNO WILKE, ANDRE BARRETTO. Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies. PARASITES & VECTORS, v. 8, DEC 15 2015. Web of Science Citations: 5.
BRUNO WILKE, ANDRE BARRETTO; MARRELLI, MAURO TOLEDO. Paratransgenesis: a promising new strategy for mosquito vector control. PARASITES & VECTORS, v. 8, JUN 24 2015. Web of Science Citations: 46.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.