Effects of seminal plasma on fertility of cryopreserved boar sperm

• Grant number: 13/01870-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2013
• End date: March 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: André Furugen Cesar de Andrade
• Grantee: Bruno Bracco Donatelli Muro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This project aims to determine if the addition/maintenance of boar seminal plasma before or after thawing improves fertility. For this, ejaculates from six boars will be collected. After analyses of raw semen, it will be diluted before centrifugation. After this, the semen pellet will be divided into two treatments: Control (CON) - suspended with extender for cryopreservation and (PAC) - suspended with extender for cryopreservation within 10 % (v/v) of seminal plasma. Extended semen will be loaded in 0.5 mL straws and then subjected to cryopreservation. Ten straws from each batch will be thawed from group PAC and ten from CON. Forty-five mL of Prolimax® extender will be added on CON and PAC groups in order to obtain fifty mL of insemination dose within 60x106sptz/mL. From the same batch, another ten straws from CON will be thawed and extended with Prolimax®, within 10 % (v/v) of seminal plasma giving rise to the third treatment, group PDC. A fertility test will be performed in seventy-five gilts. Inseminations will be performed with three treatments CON (n=25), PAC (n=25) e PDC (n=25). Fertility and birth rate will be analyzed by Chi-Square non-parametric test for P<0.05. (AU)