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Characterization of microsatellite markers for Bokermannohyla ibitiguara (Anura, Hylidae)

Grant number: 13/04023-5
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): June 17, 2013
Effective date (End): September 16, 2013
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal researcher:Cynthia Peralta de Almeida Prado
Grantee:Renato Christensen Nali
Supervisor abroad: Kelly R. Zamudio
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Research place: Cornell University, United States  
Associated to the scholarship:12/06228-0 - Genetic, morphological and acoustic diversification in populations of an anuran endemic to the Serra da Canastra, Brazil, BP.DR


Studies on landscape genetics - the combination of population genetics and landscape ecology - have increased in the past years, being very important to understand the genetic structure of populations throughout their geographical distribution. The most suitable molecular markers for this type of study are microsatellites, although their high specificity requires the development of specific markers. This project aims to characterize and optimize microsatellite markers for Bokermannohyla ibitiguara, a stream-dweller anuran endemic to the Serra da Canastra, southeastern Brazil, in the Brazilian Cerrado. We will also test cross-amplification for three other species in the genus. From an enriched partial genomic library already developed by Cornell University, NY, we will optimize at least eight loci that should match the following criteria: (1) to be polymorphic, (2) to show reliable amplification, and (3) produce clean peaks in preliminary amplification tests. DNA will be extracted from tissue samples using Chelex solution (Chelex-100, Bio-Rad) and proteinase K. Polymerase chain reactions will be followed by multiplexed groups, using a three-primer method for genotyping during electrophoresis. The optimized markers will be extremely useful, not only for landscape genetic studies of B. ibitiguara, as described in the original associated project, but also will for studies involving behavior (e.g., paternity analysis) and population genetics of other species in the genus Bokermannohyla. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NALI, RENATO C.; BECKER, CARLOS GUILHERME; ZAMUDIO, KELLY R.; PRADO, CYNTHIA P. A. Topography, more than land cover, explains genetic diversity in a Neotropical savanna tree frog. DIVERSITY AND DISTRIBUTIONS, v. 26, n. 12 AUG 2020. Web of Science Citations: 0.
NALI, RENATO C.; ZAMUDIO, KELLY R.; HADDAD, CELIO F. B.; PRADO, CYNTHIA P. A. Size-Dependent Selective Mechanisms on Males and Females and the Evolution of Sexual Size Dimorphism in Frogs. American Naturalist, v. 184, n. 6, p. 727-740, DEC 2014. Web of Science Citations: 24.
NALI, RENATO C.; ZAMUDIO, KELLY R.; PRADO, CYNTHIA P. A. Microsatellite markers for Bokermannohyla species (Anura, Hylidae) from the Brazilian Cerrado and Atlantic Forest domains. Amphibia-Reptilia, v. 35, n. 3, p. 355-360, 2014. Web of Science Citations: 3.

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