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Assessment of biological interaction between chitosan, collagen and hydroxyapatite and ovine bone tissue

Grant number: 13/00878-6
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2013
Effective date (End): September 30, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:André Luis do Valle de Zoppa
Grantee:Geissiane de Moraes Marcondes
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In human and veterinary orthopedics, are often required major repairs due to bone loss, either by surgical or pathological origin, and indispensable use of implants for aesthetic and functional correction of members. For years the sheep have been used as experimental models to human studies in orthopedics due to similarity in the weight, bone and joint and bone regeneration. There is a tendency to use the sheep studies also in veterinary medicine, especially in orthopedics equine, being an animal smaller and easier handling, and the number of animals studied can be greater, resulting in higher statistical significance. Many researchers have tried to develop and study the compatibility of different types of implants, in order to better promote repair of injured bone areas. The materials of biological origin, known as biomaterials are being used mainly in the manufacture of prostheses and orthoses. In orthopedics, the use of biomaterials to stimulate osteoinduction and / or osteoconduction in fractures with loss of bone substance still needs to find better ways to use. Natural polymers are generally biodegradable and have excellent biocompatibility compared to synthetic polymers. Chitosan, a natural biomaterial, stimulates cell growth by strong adhesion cells acquire the polymer and proliferate more rapidly. Due to the properties of biocompatibility and biodegradability, this polymer can act as a bone substitute and, over time, be replaced. The hydroxyapatite and collagen are also natural biomaterials, being used as natural extracellular matrix, serving as a support for growth of new tissue, for example bone tissue. In this research project will be used six non-pregnant ewes, will be made a failed bone circular of 7 mm in diameter with the aid of a trephine, in every bone III / IV metacarpal (proximal). Subsequently, on one of the members and random webs are implanted bone base of chitosan, hydroxyapatite and collagen, and the other member will serve as a control. Postoperatively, the animals will be evaluated following the protocol namely lameness, general physical examination, radiographs, ultrasound exams and thermography. After completing 60 days, the sheep will undergo new procedure to biopsy bone in the implant area, the fragments collected will be subjected to histological evaluations (optical microscopy and scanning electron microscopy). The objective of this study is to evaluate the process of tissue repair and behavior webs of chitosan bone, hydroxyapatite and collagen receptor in tissue from experimental bone defects in bone III / IV metacarpal sheep. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARCONDES, Geissiane de Moraes. Assessment of biological interaction between chitosan, collagen, hydroxyapatite composite and ovine bone tissue. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.

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