Bioprocess, purification and characterization of peptidases produced by fungi Eupenicillium javanicum and Myceliophthora sp. and evaluating the production of biologically active peptides

• Grant number: 12/18278-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2013
• End date: August 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Hamilton Cabral
• Grantee: Youssef Ali Abou Hamin Neto
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The peptidases are enzymes that hydrolyze polypeptides in peptides and / or amino acids. It has great importance in the global scenario of enzymes, 60% of the more enzymes sold, this prominence is due to its wide applicability in many industrial sectors, such as pharmaceuticals, cosmetics, food, leather, detergents and bioremediation. The peptidases may be obtained from animals (tissues, glands and poisons), plants (fruit, seeds and latex) and micro-organisms (fungi, yeasts and bacteria), of which the latter stand out as the main source producing peptidases about 40% of peptidases commercially available. The micro-organisms produce peptidases through fermentation bioprocesses, which may be under water or solid, may be secreted or intracellular, peptidases in this study will be produced by submerged bioprocess in which the nutrients and micro-organisms are spread uniformly in a liquid medium. The fungi used for the production of peptidases will be, Eupenicillium javanicum and Myceliophthora sp, peptidases produced by these fungi will be characterized biochemically using peptide substrates (FRET). Then the peptidases will be applied in the production of peptides by hydrolysis of proteins derived from cow's milk and egg. There are several studies, which demonstrated that these peptides made from proteins derived from milk may have biological activity, so called bioactive peptides. The bioactive peptides may exhibit antimicrobial activity, citomodulatory, immunomodulatory, hypotensive, antioxidant, opioids and others. In the present study, we also will produce peptides by hydrolysis of milk proteins and egg, these protein hydrolysates will have their peptides purified and characterized by determination of the primary sequence, then this form, the peptides will be tested for antimicrobial activity, antitumor and hypotensive .

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HAMIN NETO, YOUSSEF A. A.; DE OLIVEIRA, LILIAN C. G.; DE OLIVEIRA, ARTHUR H. C.; ROSA, JOSE C.; JULIANO, MARIA A.; JULIANO, LUIZ; RODRIGUES, ANDRE; CABRAL, HAMILTON. Determination of Specificity and Biochemical Characteristics of Neutral Protease Isolated from Myceliophthora thermophila. PROTEIN AND PEPTIDE LETTERS, v. 22, n. 11, p. 972-982, 2015. (12/50191-4, 12/18278-2, 11/06986-0, 12/24703-8)

YOUSSEF ALI ABOU HAMIN NETO; NATHÁLIA GONSALES DA ROSA GARZON; LUCIANA BARBOSA COITINHO; LAYS MARTIN SOBRAL; ANDRÉIA MACHADO LEOPOLDINO; THAÍS REGIANI CATALDI; CARLOS ALBERTO LABATE; HAMILTON CABRAL. Fungal metalloprotease generate whey-derived peptides that may be involved in apoptosis in B16F10 melanoma cells. FOOD SCIENCE AND TECHNOLOGY, v. 42, 2022-07-26. (12/18278-2, 20/14426-3, 12/24703-8)

ABOU HAMIN NETO, YOUSSEF ALI; DA ROSA GARZON, NATHALIA GONSALES; PEDEZZI, RAFAEL; CABRAL, HAMILTON. Specificity of peptidases secreted by filamentous fungi. BIOENGINEERED, v. 9, n. 1, p. 30-37, 2018. (11/06986-0, 12/18278-2)

HAMIN NETO, YOUSSEF A. A.; ROSA, JOSE CESAR; CABRAL, HAMILTON. Peptides with antioxidant properties identified from casein, whey, and egg albumin hydrolysates generated by two novel fungal proteases. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 49, n. 7, MAY 2019. (12/18278-2)

ABOU HAMIN NETO, YOUSSEF ALI; COITINHO, LUCIANA BARBOSA; PEDRO DE FREITAS, LUIS ALEXANDRE; CABRAL, HAMILTON. Box-Behnken analysis and storage of spray-dried collagenolytic proteases from Myceliophthora thermophila submerged bioprocess. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 47, n. 5, p. 473-480, 2017. (11/06986-0, 12/18278-2, 12/24703-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HAMIN NETO, Youssef Ali Abou. Bioprocess, purification and characterization of the peptidases produced by the fungi Eupenicillium javanicum and Myceliophthora thermophila and evaluation of the production of biologically active peptides. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.