This project is a continuation of our research line, that has been supported by FAPESP aiming the systematic study of the structural properties and kinetics of Na,K-ATPase from gill tissue of different species of crabs and shrimps in order to establish a model for the adaptation of crustaceans to environments of different salinities. Hemolymph osmolarity of crustaceans is maintained by the Na,K-ATPase and the adaptation mechanism suggests that the responses of crustaceans must involve a rapid activation of the transport of ions that must be primary to any other physiological responses of these animals. The current project aims to investigate the cellular signaling linked to the regulation of gill Na,K-ATPase of Cardisoma guanhumi and its direct consequences on the uptake of NaCl during acclimation to hyposaline (salinity blow 330 salinity) and marine (around 330 salinity) environments. The following studies will be undertaken: 1) preparation of gill Na,K-ATPase from Cardisoma guanhumi. 2) kinetic characterization of Na,K-ATPase modulation by ATP, PNPP, sodium, potassium, magnesium and ammonium. 3) expression of the gill proteins. 4) subcellular localization of Na,K-ATPase. 5) quantification of enzymes involved in oxidative stress in animals acclimated to different salinities. 6) study of phosphoenzyme formation. 7) study of the effect of endogenous and exogenous FXYD2 on Na,K-ATPase activity. 8) study the effect of polyamines on Na,K-ATPase activity.
News published in Agência FAPESP Newsletter about the scholarship: