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Kinetic and molecular Characterization of the gill (Na+,K+)-ATPase of crab Cardisoma guanhumi (Latreille).

Grant number: 13/00976-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2013
Effective date (End): July 31, 2017
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal researcher:Francisco de Assis Leone
Grantee:Daniel Lima de Farias
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


This project is a continuation of our research line, that has been supported by FAPESP aiming the systematic study of the structural properties and kinetics of Na,K-ATPase from gill tissue of different species of crabs and shrimps in order to establish a model for the adaptation of crustaceans to environments of different salinities. Hemolymph osmolarity of crustaceans is maintained by the Na,K-ATPase and the adaptation mechanism suggests that the responses of crustaceans must involve a rapid activation of the transport of ions that must be primary to any other physiological responses of these animals. The current project aims to investigate the cellular signaling linked to the regulation of gill Na,K-ATPase of Cardisoma guanhumi and its direct consequences on the uptake of NaCl during acclimation to hyposaline (salinity blow 330 salinity) and marine (around 330 salinity) environments. The following studies will be undertaken: 1) preparation of gill Na,K-ATPase from Cardisoma guanhumi. 2) kinetic characterization of Na,K-ATPase modulation by ATP, PNPP, sodium, potassium, magnesium and ammonium. 3) expression of the gill proteins. 4) subcellular localization of Na,K-ATPase. 5) quantification of enzymes involved in oxidative stress in animals acclimated to different salinities. 6) study of phosphoenzyme formation. 7) study of the effect of endogenous and exogenous FXYD2 on Na,K-ATPase activity. 8) study the effect of polyamines on Na,K-ATPase activity.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FARIAS, DANIEL L.; LUCENA, MALSON N.; GARCON, DANIELA P.; MANTELATTO, FERNANDO L.; MCNAMARA, JOHN C.; LEONE, FRANCISCO A.. A Kinetic Characterization of the Gill (Na+, K+)-ATPase from the Semi-terrestrial Mangrove Crab Cardisoma guanhumi Latreille, 1825 (Decapoda, Brachyura). Journal of Membrane Biology, v. 250, n. 5, p. 517-534, . (10/17534-0, 13/00976-8, 13/24252-9, 10/50188-8, 13/22605-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FARIAS, Daniel Lima de. Kinetic and molecular characterization of the (Na +, K +) - ATPase of the gill tissue of the Cardisoma guanhumi crab (Latreille, 1825).. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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