Enzymatic transformation of CO2 under continuous flow

• Grant number: 13/04540-0
• Support Opportunities: Scholarships abroad - Research
• Start date: September 01, 2013
• End date: August 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Leandro Helgueira de Andrade
• Grantee: Leandro Helgueira de Andrade
• Host Investigator: Timothy Free Jamison
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Massachusetts Institute of Technology (MIT), United States

Abstract

CO2 is a C1 building block, but it has a great importance, since it can be used for making several organic chemicals. Besides, it can be considered an economical, safe, and renewable carbon source. In this project, we will develop an enzymatic system to transform CO2 under continuous flow. Two strategies will be employed. The first one is dedicated to the co-immobilization of formate dehydrogenase (FateDH) and NADH (cofactor) on conductive supporting to be applied an electrochemical regeneration. Another strategy involves the use of a second enzyme (GDH, glutamate dehydrogenase) and NADH (cofactor). In this case, we intend to carry out the co-immobilization of both enzymes FateDH and GDH GDH (formate dehydrogenase for CO2 reduction and glutamate dehydrogenase for cofactor regeneration). All these systems will be applied to evaluate the formic acid production under continuous flow. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDRADE, LEANDRO H.; SOUSA, BRUNO A.; JAMISON, TIMOTHY F.. Confining a Biocatalyst for Highly Efficient and Selective Synthesis of Carboxamide Derivatives under Continuous-Flow Conditions. JOURNAL OF FLOW CHEMISTRY, v. 6, n. 2, p. 67-72, JUN 2016. (14/22457-5, 13/04540-0)

ANDRADE, LEANDRO H.; KROUTIL, WOLFGANG; JAMISON, TIMOTHY F.. Continuous Flow Synthesis of Chiral Amines in Organic Solvents: Immobilization of E. coli Cells Containing Both omega-Transaminase and PLP. ORGANIC LETTERS, v. 16, n. 23, p. 6092-6095, DEC 5 2014. (13/04540-0)

CORREIA, VALQUIRIO G.; ABREU, JULIANA C.; BARATA, CAIO A. E.; ANDRADE, LEANDRO H.. Iron-Catalyzed Synthesis of Oxindoles: Application to the Preparation of Pyrroloindolines. ORGANIC LETTERS, v. 19, n. 5, p. 1060-1063, MAR 3 2017. (16/05633-0, 13/04540-0, 14/22457-5)