Analysis of genetic diversity in agents of paracoccidioidomycosis using AFLP (Amplified fragment length polymorphism)

• Grant number: 13/05405-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2013
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Zoilo Pires de Camargo
• Grantee: Thiago Nunes Roberto
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 09/54024-2 - Molecular biology and proteomics of medically interesting fungi: Paracoccidioides brasiliensis and Sporothrix schenckii, AP.TEM

Abstract

The paracoccidioidomycosis (PCM) is a systemic fungal infection with high prevalence in Latin America, especially in Brazil, focusing mainly in adults, males and rural workers. The etiologic agent is a thermodimorphic fungi belonging to the genus Paracoccidioides. Up to date, phylogenetic studies of agents of PCM were able to identify two distinct species: P. brasiliensis (with three cryptic species S1, PS2 and PS3) and P. lutzii. With the development of biotechnology, a great deal of methodologies with DNA markers, including AFLP (Amplified Fragment Length Polymorphism) is available to distinguish among cryptic species. AFLP allows the detection of genetic polymorphism in the same population of organisms from the selective PCR amplification of fragments of genomic DNA digested by restriction enzymes. This study aims to analyze the genetic diversity of isolates of Paracoccidioides species from different geographical regions, through AFLP, contributing to the understanding of the epidemiology of the fungus and serving as input to further research in order to develop strategies for disease control.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROBERTO, T. N.; DE CARVALHO, J. A.; BEALE, M. A.; HAGEN, F.; FISHER, M. C.; HAHN, R. C.; DE CAMARGO, Z. P.; RODRIGUES, A. M.. xploring genetic diversity, population structure, and phylogeography in Paracoccidioides species using AFLP marker. STUDIES IN MYCOLOGY, n. 100, SEP 2021. (13/05405-9, 17/27265-5, 18/21460-3)

ROBERTO, THIAGO NUNES; RODRIGUES, ANDERSON MESSIAS; HAHN, ROSANE CHRISTINE; DE CAMARGO, ZOILO PIRES. Identifying Paracoccidioides phylogenetic species by PCR-RFLP of the alpha-tubulin gene. Medical Mycology, v. 54, n. 3, p. 240-247, MAR 2016. (11/07350-1, 13/05405-9, 09/54024-2)