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The effects of Low Intensity Laser bone repair after complete spinal cord injury in rats

Grant number: 13/06200-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2013
Effective date (End): December 31, 2014
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal researcher:Carla Christina Medalha
Grantee:Ana Lúcia Yaeko da Silva Santos
Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

Introduction: The spinal cord injury (SCI) is incapacitating, irreversible and costly economic and social. One of the main consequences of the LME is the significant loss Bone tissue within a few months to a year after trauma, leading to a decline in bone mineral density (BMD) and increased fracture risk. There is no literature standards for diagnosis, prevention and treatment of fractures after SCI. Objective: To evaluate the effects of low intensity laser therapy (LLLT; » = 830nm), fluency 120J/cm² consolidation of bone defects in tibias of rats with spinal cord injury. Methods: In this study, will be used two groups of animals being Experimental group 1 (n = 10) animals with spinal cord injury and fracture treated with LLLT and Group 2 control (n = 10) animals with spinal cord injury with defect and without laser treatment. The Rats are anesthetized and after laminectomy at the spinal level will transection T9- T10. After 2 weeks of spinal cord injury will be accomplished the bone defect. Treatment will accomplished by means of a laser treatment apparatus Portable Laser Model DMC, 830nm output power of 30 mW power density of 50 W / cm ², diameter beam of 0.6 mm, divergence of 1.5 °, the fluency of 120 J / cm ², and will begin 24 hours after surgery for bone defect and will be repeated every 48 hours for a 3 weeks. After euthanasia, will be performed complete surgical resection the tibia, and the right tibia for histological analysis and designated left tibia biomechanical analysis. The right tibia are prepared for making blades stained with HE, which will be evaluated by morphometric analysis and the analysis qualitative morphological.

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