Biomarkers of cardiomyopathy in dystrophy: a metabolomic study and pharmacological therapy

• Grant number: 12/13577-1
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2013
• Effective date (End): July 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Maria Julia Marques
• Grantee: Adriana Fogagnolo Mauricio
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In Duchenne Muscular Dystrophy (DMD) and in the mdx mice, model of DMD, lack of dystrophin leads to skeletal and cardiac muscle loss, fibrosis and progressive cardiorespiratory failure. Biomarkers for DMD have been widely investigated to follow up the evolution of the disease, facing the new pharmacological, genetics and cellular therapies to DMD. In the present study, by using metabolomic analysis of the cardiac and diaphragm muscles of the mdx, we aim to identify new biomarkers that can be correlated to heart and / or diaphragm dystrophinopathy, at later stages of the disease. We will use omega-3, an anti-inflammatory drug that had been proven effective in protecting dystrophic skeletal muscle against myonecrosis, as a tool to validate the potential new biomarkers here described. Additionally, we will investigate the effects of omega-3 in mdx cardiomyopathy. Overall, with the present study we will add a new technique (metabolomics) for the analysis of dystrophic muscle in our laboratory that we hope will help searching new molecules that can better signal the progress of the pathology in skeletal and cardiac muscles, and further support the use of omega-3 as an additional therapy. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, JULIANO ALVES; MAURICIO, ADRIANA FOGAGNOLO; MARQUES, MARIA JULIA; NETO, HUMBERTO SANTO. Dual Therapy Deflazacort/Doxycyclyne Is Better Than Deflazacort Monotherapy to Alleviate Cardiomyopathy in Dystrophin-Deficient mdx Mice. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY AND THERAPEUTICS, v. 22, n. 5, p. 458-466, SEP 2017. Web of Science Citations: 2.

MAURICIO, ADRIANA FOGAGNOLO; DE CARVALHO, SAMARA CAMACARI; SANTO NETO, HUMBERTO; MARQUES, MARIA JULIA. Effects of dietary omega-3 on dystrophic cardiac and diaphragm muscles as evaluated by H-1 magnetic resonance spectroscopy: Metabolic profile and calcium-related proteins. CLINICAL NUTRITION ESPEN, v. 20, p. 60-67, AUG 2017. Web of Science Citations: 2.

MAURICIO, ADRIANA FOGAGNOLO; PEREIRA, JULIANO ALVES; NETO, HUMBERTO SANTO; MARQUES, MARIA JULIA. Effects of fish oil containing eicosapentaenoic acid and docosahexaenoic acid on dystrophic mdx mice hearts at later stages of dystrophy. NUTRITION, v. 32, n. 7-8, p. 855-862, JUL-AUG 2016. Web of Science Citations: 5.

MARANHAO, JULIANA BARROS; MOREIRA, DRIELEN DE OLIVEIRA; MAURICIO, ADRIANA FOGAGNOLO; DE CARVALHO, SAMARA CAMACARI; FERRETTI, RENATO; PEREIRA, JULIANO ALVES; SANTO NETO, HUMBERTO; MARQUES, MARIA JULIA. Changes in calsequestrin, TNF-alpha, TGF-beta and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles. International Journal of Experimental Pathology, v. 96, n. 5, p. 285-293, OCT 2015. Web of Science Citations: 7.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MAURICIO, Adriana Fogagnolo. Biomarkers of cardiomyopathy in muscle dystrophy : metabolomics and therapy with omega-3. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia.