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Quantification of proviral load in groups with different results for the HTLV-1

Grant number: 13/03086-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2013
Effective date (End): December 31, 2013
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Sabri Saeed Mohamed Ahmed Al-Sanabani
Grantee:Jaqueline Tomoko Watanabe
Home Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

HTLV-1 is etiologically associated with various disorders including adult T-cell leukaemia/lymphoma (ATL) and HTLV-I Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP). The proviral load is an important biological marker for understanding the pathogenesis of the virus and for shedding light on whether or not the virus is correlated to disease progression. In this project, we aimed to quantify the HTLV-1 proviral load in the peripheral blood mononuclear cells (PBMCs) from 250 asymptomatic HTLV-1 carriers, 10 with HTLV-I HAM-TSP and 7 patients with ATL. The levels of the proviral load will be determined by a real time PCR. The results of this study will allow us to classify the asymptomatic carriers into low, intermediate and high proviral load groups. These results are expected to pave the way for the main project that aim to study the regulatory role of miRNAs in individuals with and without symptoms of HTLV-1, asymptomatic carriers with low and high proviral load with or without T cells expansions.