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Palmitoleic acid supplementation effects on immune-metabolics alterations induced by high fat diet on PPAR knockout mice

Grant number: 13/04765-1
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2013
Effective date (End): December 04, 2017
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal Investigator:José Cesar Rosa Neto
Grantee:Camila Oliveira de Souza
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Obesity and insulin resistance are related to glucose and fat acids metabolism hepatic alterations. The insulin resistance promotes transcription factors activation that elevates gluconeogenesis and fatty acids de novo hepatic production, inhibit glycogen synthesis and reduces glucose and fatty acids oxidation. This effect enhances free fatty acids circulation levels and induces triacylglycerols ectopic deposition on hepatic parenchyma, impairing obese patient healthy quality. Recent studies demonstrate that palmitoleic acid (or palmitoleate) have anti-inflammatory properties and operates on muscle and liver as adipokine, which improve insulin resistance and enhance liver lipids oxidation. These effects could be associates to PPAR-alpha, a nuclear receptor that inhibits pro-inflammatory pathways and enhances mitochondria and particularly peroxisome beta-oxidation. Thus, our objective are investigate palmitoleate supplementation effect, as well as PPAR-alpha influence on liver inflammatory process, on high fat diet insulin resistance and on hepatic lipids synthesis and oxidation. Therefore, we will investigate in C57BL/6J and PPAR-alpha knockout (PPAR-alpha(-/-) mice the palmitoleate supplementation effects on peripheral and hepatic insulin resistance, we will determine the palmitoleic acid effect on liver inflammatory process, will evaluate lipids hepatic metabolism by the novo synthesis, mitochondrial and peroxisomal beta-oxidation, krebs cycle and electron transport chain, finally, we will confect liver histological slices to determinate inflammatory cells and the accumulation of lipids in the liver,verifying palmitoleate effect on hepatic steatosis. Therefore, this project will be important to understand the mechanisms involved in liver palmitoleic acid action, elucidating obesity, diabetes mellitus type 2 and associates conditions related questions. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE SOUZA, CAMILA O.; TEIXEIRA, ALEXANDRE A. S.; BIONDO, LUANA A.; LIMA JUNIOR, EDSON A.; BATATINHA, HELENA A. P.; ROSA NETO, JOSE C.. Palmitoleic Acid Improves Metabolic Functions in Fatty Liver by PPAR-Dependent AMPK Activation. Journal of Cellular Physiology, v. 232, n. 8, p. 2168-2177, . (16/01409-8, 13/04765-1)
DE SOUZA, CAMILA OLIVEIRA; VALENZUELA, CARINA A.; BAKER, ELLA J.; MILES, ELIZABETH A.; NETO, JOSE C. ROSA; CALDER, PHILIP C.. Palmitoleic Acid has Stronger Anti-Inflammatory Potential in Human Endothelial Cells Compared to Oleic and Palmitic Acids. MOLECULAR NUTRITION & FOOD RESEARCH, v. 62, n. 20, . (13/04765-1, 15/06376-8, 16/01409-8)
SOUZA, CAMILA O.; TEIXEIRA, ALEXANDRE A. S.; BIONDO, LUANA AMORIM; SILVEIRA, LOREANA SANCHES; DE SOUZA BREDA, CRISTIANE N.; BRAGA, TARCIO T.; CAMARA, NIELS O. S.; BELCHIOR, THIAGO; FESTUCCIA, WILLIAM T.; DINIZ, TIEGO A.; et al. Palmitoleic acid reduces high fat diet-induced liver inflammation by promoting PPAR-gamma-independent M2a polarization of myeloid cells. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, v. 1865, n. 10, . (13/04765-1, 19/06172-4, 19/24112-9, 15/19530-5, 16/01409-8)
SILVEIRA, LOREANA S.; PIMENTEL, GUSTAVO D.; SOUZA, CAMILA O.; BIONDO, LUANA A.; TEIXEIRA, ALEXANDRE ABILIO S.; LIMA, EDSON A.; BATATINHA, HELENA A. P.; ROSA NETO, JOSE C.; LIRA, FABIO S.. Effect of an acute moderate-exercise session on metabolic and inflammatory profile of PPAR- knockout mice. Cell Biochemistry and Function, v. 35, n. 8, p. 510-517, . (13/09367-4, 14/01246-6, 13/10861-3, 13/04765-1, 13/25310-2)
SOUZA, CAMILA O.; TEIXEIRA, ALEXANDRE A. S.; BIONDO, LUANA A.; SILVEIRA, LOREANA S.; CALDER, PHILIP C.; ROSA NETO, JOSE C.. Palmitoleic acid reduces the inflammation in LPS-stimulated macrophages by inhibition of NF kappa B, independently of PPARs. Clinical and Experimental Pharmacology and Physiology, v. 44, n. 5, p. 566-575, . (16/01409-8, 13/04765-1)
DE SOUZA, CAMILA O.; VANNICE, GRETCHEN K.; ROSA NETO, JOSE C.; CALDER, PHILIP C.. Is Palmitoleic Acid a Plausible Nonpharmacological Strategy to Prevent or Control Chronic Metabolic and Inflammatory Disorders?. MOLECULAR NUTRITION & FOOD RESEARCH, v. 62, n. 1, . (16/01409-8, 13/04765-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Camila Oliveira de. The role of PPARs on the immuno-metabolic benefits caused by palmitoleic acid.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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