TUMORIGENIC FIBROBLAST INTERACTION IN CO-CULTURE WITH MAMMARY TUMOR CELLS IN RESPONSE AND THE THERAPEUTIC USE OF MELATONIN

• Grant number: 13/07546-9
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2013
• Effective date (End): February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Debora Aparecida Pires de Campos Zuccari
• Grantee: Larissa Bazela Maschio
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

The breast neoplasms are the most common tumors in women and female dogs. The study of mechanisms controlling tumor progression has evolved in recent years, and a growing number of strategies have served as a tool for further research. Cancer-associated fibroblasts (CAFS) are the most abundant cells in the tumor microenvironment, able to mediate inflammatory responses through the secretion of chemokines, interleukins and growth factors and thus act in differentiation and tumor progression. The hormone melatonin has been associated with various mechanisms of action and oncostatics and oncoprotectors effects, besides reduced the synthesis of surrounding fibroblasts and endothelial cells in breast cancer by regulating cytokines present in the tumor microenvironment. The objective of this study is to evaluate the interaction of CAFS in co-culture with lineages of human and canine metastatic breast cancer to verify the differential expression of proteins related to angiogenesis, apoptosis and differential cytokine profile before and after treatment with melatonin. Will be performed a melatonin treatment in co-cultivation of B231 and MDA-P-114 strains with CAFS and trace a profile of the main factors involved in the processes of angiogenesis, apoptosis and cytokines through the techniques of Antibodie Array. These results could facilitate the interaction of tumor microenvironment with tumor growth associated with the use of melatonin as therapeutic agents in breast cancer.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MASCHIO-SIGNORINI, LARISSA B.; GELALETI, GABRIELA B.; MOSCHETTA, MARINA G.; BORIN, THAIZ F.; JARDIM-PERASSI, BRUNA V.; LOPES, JULIANA R.; LACERDA, JESSICA Z.; ROELA, ROSIMEIRE A.; BORDIN, NEWTON A.; CORREA, LIZANDRA A.; et al. Melatonin Regulates Angiogenic and Inflammatory Proteins in MDA-MB-231 Cell Line and in Co-culture with Cancer-associated Fibroblasts. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, v. 16, n. 11, p. 1474-1484, 2016. (13/07546-9)