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Identification of metabolites of study remobilization of bound residues herbicide in soil cultivation of cane sugar with application the vinasse, straw cane and pie filter

Grant number: 13/10018-4
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): July 05, 2013
Effective date (End): November 04, 2013
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Valdemar Luiz Tornisielo
Grantee:Marcela Lembi Viti
Supervisor abroad: María Teresa Martínez Soria
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Local de pesquisa : Universidad de La Rioja (UR), Spain  
Associated to the scholarship:11/15677-0 - Remobilization of bound residues of herbicides in soils cultivated with sugar cane with vinasse application, straw and filter cake, BP.MS

Abstract

The objective of this project is to identify the metabolites formed from remobilization and degradation of bound residues of 14C - diuron, 14C - 14C and hexazinone - metribuzin in two soils of growing sugar cane, with application of vinasse treatments , filter cake and straw cane sugar. Through analysis by TLC (TLC), it was observed that there was formation of metabolites of herbicides and these will be identified by UPLC/Q-TOF-MS and NMR spectroscopy. The efficiency ultra performance liquid chromatography (UPLC) is considered the latest advancement in science and analytical separation using stationary phases with particles smaller than 2 micrometers. The use of these small particles with high mobile phase linear velocity increases the resolution and detection and reduces the analysis time. Thus, the analysis is faster the volume of solvent is smaller, the efficiency is higher and the detection is 2 to 3 times greater when compared to HPLC analysis. The mass spectrometer Q-TOF is extremely fast, has a high resolution, sensitive techniques that tolerate undesirable substances and allows the identification of metabolites without the use of standards. The Nuclear Magnetic Resonance Spectroscopy finds applications in various fields of science, identifying components of complex mixtures and providing detailed information on the structure, dynamics, reaction state and chemical environment of molecules. (AU)