Development of polymeric nanostructures for application in tissue engineering

• Grant number: 13/03474-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2013
• Effective date (End): September 23, 2017
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Luiz Henrique Capparelli Mattoso
• Grantee: Rafaella Takehara Paschoalin
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated scholarship(s): 15/00771-2 - Immunological study of the interaction between dendritic cells an biopolymeric nanofibers, BE.EP.DR

Abstract

This project aims the study of nanostructured polymeric membranes interaction with animal stem cell, correlating the materials properties and their biological response. In this work, will be characterized the Polylactic acid (PLA) polymeric membranes and their polyethyleneglycol (PEG) blend, which will be produced by solution blow spinning technique (SBS). It will be evaluated the adhesion, proliferation and viability of the steam cells correlating them with the surface properties of the nanofibers such as, wettability, roughness, surface area, etc. The development of a methodology for porous tridimensional supports (scaffolds) to cells growing aims to facilitate the formation of tissues and functional organs which will be used in regenerative veterinary medicine. The present project may provide unprecedented results on the application of nanostructured polymeric membranes based in biodegradable polymers on systems to adhesion of animals stem cell, since this issue is rarely cited in the literature. Furthermore, the nanostructures will be obtained by a potential large scale production technique and in situ viability. The project works at the interface of nanotechnology, chemistry and engineering of polymeric materials, aiming the practical applications of these membranes in tissue engineering.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANZOLI, ALEXANDRA; STEFFENS, CLARICE; PASCHOALIN, RAFAELLA TAKEHARA; GRABOSKI, ADRIANA MARCIA; BRANDAO, HUMBERTO DE MELLO; DE CARVALHO, BRUNO CAMPOS; BELLINI, JOS LUIZ; HERRMANN JR, PAULO SERGIO DE PAULA. Volatile compounds monitoring as indicative of female cattle fertile period using electronic nose. SENSORS AND ACTUATORS B-CHEMICAL, v. 282, p. 609-616, MAR 1 2019. (13/03474-3)

PASCHOALIN, RAFAELLA T.; TRALDI, BRUNA; AYDIN, GUELCAN; OLIVEIRA, JULIANO E.; MITTEN, STEPHAN; MATTOSO, LUIZ H. C.; ZENKE, MARTIN; SECHI, ANTONIO. Solution blow spinning fibres: New immunologically inert substrates for the analysis of cell adhesion and motility. Acta Biomaterialia, v. 51, p. 161-174, MAR 15 2017. (15/00771-2, 13/03474-3)