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Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro

Grant number: 12/17236-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2013
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Fernanda Lourenção Brighenti
Grantee:Rosa Virginia Dutra de Oliveira
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):14/02397-8 - Interactions between cariogenic bacteria: susceptibility to chlorhexidine and Confocal Scanning Laser Microscopy using drip-flow biofilm reactor, BE.EP.DR

Abstract

The proximity between microorganisms present in dental biofilm determines the existence of interaction between them, which can benefit the involved bacteria. This study aims to evaluate the interaction between Streptococcus mutans ATCC 35688 with two other cariogenic microorganisms: Actinomyces naeslundii ATCC 19039 and Streptococcus sobrinus ATCC 33478, in biofilms formed in vitro. First, biofilms growth will be standardized considering bacterial concentration (106, 107 and 108 UFC/ml) and growth period (24, 48 and 72 h). Biofilms will grow in triplicate on the surface of glass discs in BHI supplemented with 0.2% (v/v) sucrose, using a model of active attachment. The number of CFU/mL of each bacteria will be obtained with a stereomicroscope after plating in selective and/or differential culture media. Next, the metabolic activity (XTT test), the biochemical composition (biomass and production of proteins, carbohydrates and lactic acid) will be evaluated. To evaluate the susceptibility to chlorhexidine, biofilms will grow in a drip-flow reactor. The immediate effect of chlorhexidine and re-growth after 24 h will be assessed by confocal microscopy. Data of microbiology, metabolic and biochemical tests will be statistically analysed using the software GraphPad Prism version 3.02 considering the significance level of 5%. The confocal microscopy images will be analysed using COMSTAT. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROSA VIRGINIA DUTRA DE OLIVEIRA; YASMIN ETIENNE ALBUQUERQUE; DENISE MADALENA PALOMARI SPOLIDORIO; CRISTIANE YUMI KOGA-ITO; ELISA MARIA APARECIDA GIRO; FERNANDA LOURENÇÃO BRIGHENTI. Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study. Rev. odontol. UNESP, n. ahead, p. 0-0, 2016.
ROSA VIRGINIA DUTRA DE OLIVEIRA; YASMIN ETIENNE ALBUQUERQUE; DENISE MADALENA PALOMARI SPOLIDORIO; CRISTIANE YUMI KOGA-ITO; ELISA MARIA APARECIDA GIRO; FERNANDA LOURENÇÃO BRIGHENTI. Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study. Rev. odontol. UNESP, v. 45, n. 2, p. 90-96, Abr. 2016.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
OLIVEIRA, Rosa Virginia Dutra de. Interação entre bactérias cariogênicas. 2016. 72 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Odontologia (Campus de Araraquara)..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.