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Lasertherapy effects on muscle repair process in rats with chronic alcohol ingestion

Grant number: 13/08805-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2013
Effective date (End): December 31, 2013
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal Investigator:Ana Claudia Muniz Renno
Grantee:Rafael Moraes Thomaz
Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

High level alcohol consumption in patients with traumatic injuries is associated with longer hospital stays and admission to the intensive care unit after injury, higher injury severity scores, and an increased mortality rate. These findings have multifactorial basis, but they have been attributed in large part to alcohol-associated modulation of host-defense systems and the immune response. While acute consumption is inhibitory for inflammation, chronic consumption seems to increase proinflammatory cellular response. The inflammation associated with muscular injury is necessary to remove the injured tissue and can promote regeneration by the interaction with myoblasts which promote myogenic differentiation. Low-level laser therapy effectively decrease post injury inflammatory process, stimulate new blood vessel formation and accelerate soft tissue healing. This therapeutic modality has demonstrated to improve muscle repair by inducing rapid vascularization on injury area, muscle fiber regeneration, parallel orientation of regenerated myofibers and increase tissue repair by accelerating the membrane reactions begins, increasing macrophage, fibroblasts and lymphocytes activity. The aim of this study include analyze the effects of low level laser therapy on muscle repair process in rats with chronic alcohol ingestion . Forty-eight Wistar male rats, age average 60 days, will be divided in three groups: injury (n=16), injury+alcohol (n=16), injury+alcohol+laser (n=16). Each group will be subdivided in two groups of eight animals for analyzes 3 day post-injury (n=24) and 7 days post-injury (n=24). Alcohol administration will be with two bottle choice drinking paradigm, with access to one bottle of water and other with alcohol solution 20%, 3 days per week for 45 days. Muscle injury will be induced by cryolesion on right tibial anterior muscle and laser treatment will be started immediately post-injury. Transversal hystological sections will be stained for qualitative analysis of inflammatory process, granulation tissue, necrosis area and tissue structure. The immunohystochemical analysis will be performed with objective of verify myoD expression, myogenin, COX-2 and VEGF. Comparison between quantitative results of treatment and control group will be performed by analysis of variance (ANOVA), and the Bonferroni post-test.