Differential expression of genes related of meat tenderness in Nellore cattle

• Grant number: 13/09190-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2013
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal Investigator: Lucia Galvão de Albuquerque
• Grantee: Larissa Fernanda Simielli Fonseca
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 09/16118-5 - Genomic tools to genetic improvement of direct economic important traits in Nelore cattle, AP.TEM

Abstract

Brazil is the second largest exporter of beef in the world, however, more than 80% of the meat is zebu, and needs improvement in organoleptic characteristics as color, flavor, juiciness and especially tenderness. The tenderness can be measured by sensory analysis or by measuring the shear force. This method use an apparatus who measure the force required to shear a cross section of meat and the higher the strength dispensed, lower is the tenderness showed by the cut of meat. However, these methods can only be performed after the animal was killed, and using transcriptome analysis techniques, as RNAseq, is an alternative to evaluate this feature early. Thus, the objective of this work is to identify differentially expressed genes using the technique of next generation sequencing, RNAseq, in Nellore cattle in order to produce higher quality meat. For this, will be sequenced RNA samples extracted from Longissimus dorsi muscle collected from 42 animals divided in two groups for extreme shear force and then will be analysis of differential gene expression between the two groups. Later, five differentially expressed genes will be selected and validation of expression will be performed by qRT-PCR technique. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

SIMIELLI FONSECA, LARISSA FERNANDA; DOS SANTOS SILVA, DANIELLY BERALDO; JOVINO GIMENEZ, DANIELE FERNANDA; BALDI, FERNANDO; FERRO, JESUS APARECIDO; LOYOLA CHARDULO, LUIS ARTUR; DE ALBUQUERQUE, LUCIA GALVAO. Gene expression profiling and identification of hub genes in Nellore cattle with different marbling score levels. Genomics, v. 112, n. 1, p. 873-879, JAN 2020. (09/16118-5, 13/09190-7)

SIMIELLI FONSECA, LARISSA FERNANDA; JOVINO GIMENEZ, DANIELE FERNANDA; DOS SANTOS SILVA, DANIELLY BERALDO; BARTHELSON, ROGER; BALDI, FERNANDO; FERRO, JESUS APARECIDO; ALBUQUERQUE, LUCIA GALVAO. Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness. BMC Genomics, v. 18, DEC 4 2017. (09/16118-5, 13/09190-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FONSECA, Larissa Fernanda Simielli. Differential gene expression in Nellore cattle muscle tissue divergently ranked on meat quality. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.