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Evaluation of the role of cardiac SERCA2a in rats submitted to supravalvar aortic stenosis with heart disfunction

Grant number: 13/09830-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2013
Effective date (End): August 31, 2015
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Antonio Carlos Cicogna
Grantee:Izabelle Ferreira da Silva Mazeto
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In conditions of pressure overload, the heart undergoes an adaptive process of cardiac remodeling (CR). Among the ways this modification presents itself, ventricular hypertrophy stands out. It initially determines an improvement in systolic function, seeking to maintain heart stability. However in the long term the CR represents a risk factor for ventricular dysfunction and heart failure. Among the available experimental models used to study the effects of pressure overload, there is the Supravalvar Aortic Stenosis (SVAoS) which, due to its progressive characteristics, resembles the most the equivalent condition in the human being. In this model, several authors have detected diastolic dysfunction in rats 6 weeks after the surgery. Other researchers have also found a reduction in the expression of SERCA2a. However, studies, including one in our laboratory, have found no objective relation between the decrease of SERCA2a and the reduced diastolic performance in rats with 6 weeks of aortic stenosis. Therefore, this project's goal is: 1) to evaluate the presence of heart dysfunction on the left ventricle and myocardium 18 weeks after SVAoS induction in rats, using echocardiogram e isolated papillary muscle, respectively; 2) to analyze the participation of SERCA2a in the process of heart dysfunction, by blocking this protein on the isolated papillary muscle. (AU)