Endothelial progenitor cells were first described by Asahara et al, 1997, and were isolated from humam peripheral blood. These cells are originated from bone marrow and can be characterized by the expression of CD34, CD133, VEGFR2 e CD31, CD144, Tie-2, LDL internalization and formation of tube like structures in vitro. They can be mobilized from bone marrow to peripheral blood going to the lesion site, differentiating into endothelial cells (EC) and promoting re-endothelization. They can also secrete molecules that inhibit smooth muscle cells proliferation and simulates adult endothelial cells proliferation. Arterial and venous thrombosis can lead to vascular diseases like deep vein thrombosis and cardiac diseases. Recanalization of the thrombus is a part of the vascular bead recovery after lesion and is directly linked to the cells and molecules associated to the thrombus. In this work we will obtain endothelial progenitor cells from mice bone marrow, expand then in culture for 30 days in a differentiation medium and verify their EPC characteristics. These cells are going to be tested in a carotid arterial thrombosis model and a jugular vein thrombosis model using ferric chloride in atherosclerotic mice. We are going to analyze their role in thrombus resolution and in the re-endothelization of lesioned vessel wall after injury. We hope to determine their influence in the proliferation of adult endothelial cells and in the inhibition of proliferation of smooth muscle cells that can participate in the restenosis process and remodeling of the vessel wall, studing also the role of atherosclerosis in this process.
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