Incorporation of magnesium and/or zinc doped-tricalcium phosphate ceramics doped in a hydrogel-drug matrix for osteomyelitis treatment associated with bone repairment

• Grant number: 12/23803-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2013
• Effective date (End): March 31, 2015
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Juliana Marchi
• Grantee: Karen Cristina Kai
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

Osteomyelitis is a bacterial bone infection frequently occurring in various traumas. Conventional treatment of chronic osteomyelitis is bone and adjacent soft tissue debridement with infection, associated with antibiotic treatment. In this work,we propose the development of an injectable material based in the poloxamer 407 (PL407) - alone or in combination with poloxamer 188 (PL188) - hydrogel with the antibiotic teicoplanin and different compositions beta-tricalcium phosphate (beta-TCP) doped with magnesium and/or zinc for the treatment of osteomyelitis associated with bone repair. The TCP ceramics were synthesized and characterized physically, chemically and biologically during Masters studies by Karen Cristina Kai using the techniques of X-ray diffraction, dilatometry, differential thermal analysis, specific surface area, particle diameter distribution, scanning electron microscopy, geometric and apparent density, linear shrinkage and cytotoxicity. There was also a pilot osteogenic differentiation study with pure beta-TCP. From the results obtained, the materials have been chosen with the best features for incorporation into hydrogel-drug matrix. The material characterization will be performed by the structure analysis of the systems (hydrodynamic radius and polydispersity measures), the sol-gel transition characterization (determination of gelation temperature), rheological analysis and characterization of the sol-gel transition by Differential Scanning Calorimetry (DSC), evaluation of in vitro release profile and permeation assays using pig ear skin as a model barrier, solubility assays and determination of the drug-micelle partition coefficient. The biological characterization tests will be carried by in vitro bioactivity, bacterial susceptibility, indirect cytotoxicity, cell migration and osteogenic differentiation.