Chemical study and evaluation of antimicrobial activity of endophytic fungi associated with Paepalanthus chiquitensis (Eriocaulaceae) and effects of chemical epigenetic modulators in the cultivation of Pochonia chlamydosporia

• Grant number: 13/12564-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2013
• End date: January 23, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Lourdes Campaner dos Santos
• Grantee: Felipe Hilário
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 14/25181-0 - Epigenetic modifiers: exploring the production of new natural products from endophytic fungi, BE.EP.MS

Abstract

The current project is the study of the secondary metabolites active produced by endophytic fungus associated from aerial parts of Paepalanthus gi- ganteus (Eriocaulaceae. There is more than two decades our team have been studying species of this family, focused in the isolation by chroma- tography methods like gel permeation chromatography, silica gel column chromatography, HPLC, HSCCC. More than two dozen of species already were studied and its metabolites eluci- dated by spectrometric and spectroscopic methods (MS, IR, UV, NMR). Therefore, other perspective that still does not addressed with Eriocaulaceae is the study of endophytic fungus associated with species of this family. Seeing the current impor- tance and relevance of natural products from fun- gus, we are implementing in our team the chemis- try study of endophytic fungus associated from Eriocaulaceaes in collaboration with the group led by Prof. Dr. Angela Regina Araújo (NUbbe- IQ - Unesp, Araraquara). The knowledge of the secondary metabolites from endophytic fungus complement notably the wide phytochemical work whose our team has been doing for this years. The purpose under- graduate research project will be to accomplish the cultivation of the endophytic fungus associa- tes from P. giganteus, to prepare the extracts from fungus and the relative fingerprint by HPLC-PDA and by HPLC-ESI-IT-MS of each extract. For the compounds already known will be identified with co-injections of standard and metabolite, does not isolate and does not identify should be realized fractionation and purifications by chromatography methods.Parallel will perform the antimicrobial assays of extracts, fractions and pure substances.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE AMORIM, MARCELO R.; HILARIO, FELIPE; SANO, PAULO T.; BAUAB, TAIS M.; DOS SANTOS, LOURDES C.. Antimicrobial Activity of Paepalanthus planifolius and its Major Components against Selected Human Pathogens. Journal of the Brazilian Chemical Society, v. 29, n. 4, p. 766-774, APR 2018. (13/12564-6, 15/04899-3, 13/25432-0)

FELIPE HILÁRIO; VANESSA M. CHAPLA; ANGELA R. ARAUJO; PAULO T. SANO; TAÍS M. BAUAB; LOURDES C. DOS SANTOS. Antimicrobial Screening of Endophytic Fungi Isolated from the Aerial Parts of Paepalanthus chiquitensis (Eriocaulaceae) Led to the Isolation of Secondary Metabolites Produced by Fusarium fujikuroi. Journal of the Brazilian Chemical Society, v. 28, n. 8, p. 1389-1395, 2017-08-00. (13/12564-6, 13/25432-0, 15/04899-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HILÁRIO, Felipe. Chemical study and evaluation of antimicrobial activity of endophytic fungi associated with Paepalanthus chiquitensis (Eriocaulaceae) and effects of chemical epigenetic modulators in the cultivation of Pochonia chlamydosporia. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.