Functional study of cellular immunity in brazilians with type 1 diabetes at different stages of the disease and its integration with humoral markers

• Grant number: 12/24667-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2013
• End date: October 31, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Leonilda Maria Barbosa dos Santos
• Grantee: Daniela Franchi Pereira da Silva Camilo
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The type 1 diabetes, autoimmune is the most common chronic diseases of childhood and adolescence, with grave prognosis if treated inadequately. There is heterogeneity in its clinical expression, depending on the extent of the destruction of pancreatic beta cells by autoreactive T lymphocytes. The presence of autoantibodies and genotype predisposing (HLA class II) allows the diagnosis of the disease. An autoimmune process relapsing-remitting type was proposed, involving the interference of viral infections in the loss of beta cells, gradually, but not linear. The spontaneous remission, "honeymoon", clinically detected around 50% of children with type 1 diabetes, it is timely to study immunological events. Focusing immunosuppression physiological extensively studied in experimental models, different populations of dendritic cells influence the onset of autoimmunity by the expansion of regulatory T cells by stimulation via IL-10, TGFß, indoleamine 2,3-dioxygenase (IDO). In vitro, IDO induces the expression of HLA-G, HLA class I molecule nonclassical in human dendritic cells, and both molecules cooperate to immunosuppression. However, there are few tools to identify cells that mediate the disease in humans. The objective of the study is to identify, in peripheral blood of patients with type 1 diabetes, the expression of HLA-G, IDO, as well as other markers of activated T cells, B and dendritic. An individual assessment at different stages of the disease will be included when possible: at diagnosis, remission and subsequent developments. The data will be associated with humoral markers.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMILO, DANIELA S.; PRADELLA, FERNANDO; PAULINO, MARIA FERNANDA; BARACAT, EMILIO C. E.; MARINI, SOFIA H.; GUERRA, JR., GIL; PAVIN, ELIZABETH J.; PARISI, CANDIDA; LONGHINI, ANA LEDA F.; MARQUES, SILVIA B.; et al. Partial remission in Brazilian children and adolescents with type 1 diabetes. Association with a haplotype of class II human leukocyte antigen and synthesis of autoantibodies. PEDIATRIC DIABETES, v. 21, n. 4, p. 606-614, JUN 2020. (12/24667-1, 16/17404-5, 13/12109-7, 12/04565-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAMILO, Daniela Franchi Pereira da Silva. Partial remission in Brazilian children and adolescents with type 1 diabetes: association with a haplotype of class II human leukocyte antigen and synthesis of autoantibodies. 2019. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.