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Biocompatibility and biomineralization assessment of experimental sealers compared to Pro-Root MTA®: study in the subcutaneous tissue of rats

Grant number: 13/08335-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2013
Effective date (End): August 31, 2014
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Luciano Tavares Angelo Cintra
Grantee:Marcela Ito Rey
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Among the factors that interfere with the healing process of periapical tissues after performing a root canal treatment, there is retrograde filling or root-end filling material, which by being in close contact with the periapical tissues, plays a fundamental role in the repair process of that region. Thus, an essential property that is biological compatibility, since not observed, can wake the occurrence of a series of pathological events unfavorable. In addition to biocompatibility, some materials have the ability to participate in the repair process leading to mineralization. One of the most practical and used for the study of tissue response to endodontic sealers is the implantation of polyethylene tubes containing these materials in the subcutaneous tissue of rats. Given the development of experimental cement, which contains calcium hydroxide in its formulation, we propose to evaluate quantitatively and qualitatively the tissue response to these sealers, comparing them to the Mineral Aggregate Trióxidos, Pro-Root MTA ®. Will be used 140 polyethylene tubes and 35 rats (four tubes per rat) divided into 4 groups: G1 - control (empty tubes), G2 - tubes containing SealAta; G3 - tubes containing SealAta Plus; G4 - tubes containing the ProRoot ® MTA. The evaluation times are 7, 15, 30, 60, and 90 days. After each postoperative period, seven animals are sacrificed and the polyethylene tubes together with the surrounding tissue are removed, fixed in 10% formalin, and processed for light microscopy analysis, with inclusion in glycol methacrylate, cut serial 3¼m and stained with hematoxylin and eosin and serial sections of 10¼m and Von Kossa staining and without staining under polarized light for analysis. The tissue sections will be evaluated by pre-established criteria by assigning scores to the microscopic events observed. Data will be analyzed from a statistical viewpoint using the Kruskal Wallis and Dunn with a significance level of 5% (p <0.05). (AU)

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