Cystic fibrosis (CF) is one of the fateful autossomal recessive illnesses most common at occidental world. Chronic infection of respiratory tract accounts for the high rate of morbidity and mortality of patients suffering from cystic fibrosis. Achromobacter and Burkholderia cepacia complex (BCC) bacteria are among the gram-negative non-fermenting (BGN-NF) more isolated from these patients. Due to phenotypic similarity between them, misidentifications are often reported, making essential molecular methods for identification. These pathogens are responsible for severe inflammation, with progressive decrease of lung function, and also show high resistance to multiple antibiotics It is a challenge to understand the epidemiology, ecology and clinical impact of human infection with Achromobacter, therefore it is extremely important to perform methods as molecular typing for increasing epidemiological knowledge of these species. The purpose of this work is to study Achromobacter and BCC bacteria, in CF patients being treated at the Hospital das Clínicas of the Ribeirão Preto Medical School of the University of São Paulo - USP (HCFMRP-USP) and Hospital das Clínicas of Medical Sciences School - UNICAMP (HCFCM-UNICAMP): identify BCC genomovares and investigate virulence genotypes; evaluate the antimicrobial susceptibility from isolates belonging to Achromobacter sp. and Burkholderia sp.; investigate possible genetic relationships among isolates of A. xylosoxidans and A. denitrificans, using macro restriction analysis of chromosomal DNA after Pulsed-field Gel electrophoresis; elucidate the taxonomy and epidemiology of isolates from Achromobacter sp. by Multilocus Sequence Typing (MLST). The results will be discussed with the medical team involved in order to provide life improved quality of these patients.
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