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Therapeutic efficacy study, clinical and ecotoxicological safety of the Fosfomicine antibiotic, administered by oral, for Aeromonas hydrophila and Streptococcus agalactiae treatment in Nile Tilapia, Oreochromis niloticus

Grant number: 13/11675-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2013
Effective date (End): February 28, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal Investigator:Marco Antonio de Andrade Belo
Grantee:Roberto Barbuio
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


Present research has how aim to evaluate the therapeutic efficacy, clinic and ecotoxicological safety of the fosfomicine antibiotic, administered by oral to add in the ration, for Aeromonas hydrophila and Streptococcus agalactiae treatment in Oreochromis niloticus. For this, six experiments will be performed: The fosfomicine safety will be to evaluate in amount dose-answer study, evaluating the clinic safety in dose 40, 80 e 160 mg fosfomicine/kg l.w. (this is equal at 2000, 4000 e 8000 g fosfomicine/ton of feed, respectively). Efficacy study with the therapeutic dose 40 mg fosfomicine/kg l.w. will carry out in challenged fish with live inocula of A. hydrophila and S. agalactiae. In the ecotoxicology tests will determine the lethal concentration (LC50;48h) to tilapia (O. niloticus), aquatic plants (Lemna minor), crustacean (Daphnia magna) and snail (Pomacea canaliculata ). In the clinical safety will be use 130 and 170 Nile tilapias (± 200 g), from the same spawning, which will be to add in 13 and 17 aquariums of 250 L each one (n=10) respectively, constituting the different treatments: in the clinical study (T0= no treated; T1, T2 and T3= treated with 40, 80 and 160 mg/kg l.w., respectively) and in the therapeutic study (T4= no challenged and no treated; T5= infected with A.hydrophila, no treated; T6= A. hydrophila and treated; T7= infected with S. agalactiae, no treated; T8= S. agalactiae and treated). In the clinical study, the fish will be to feed with fosfomicine ration, being anesthetize and sampled 48, 96, 192 and 288 hours after exposure. In the therapeutic study, the fish will anesthetize for bacterial challenge 24 hours after start the fosfomicine treatment and they will treat for seven days (T6 and T8), being these fish sampled and anesthetize 48, 96, 192 e 288 hours pos-challenge. After the end the treatment will perform the microbiological study from the fish tissues in the attempt to isolate the pathogens. The animals will evaluate daily about behavior, clinical signs and mortality. The blood samples will use in the complet hemogram study and serum biochemical. It will collect liver, spleen, kidney, gill and gastric mucosa tissues for histopathological study. Thus, the correlation among the studies: clinical-behavior, hematology, biochemical, histopathology and somatic will provide important subsidies in the clinical safety comprehension and investigation and of the fosfomocine efficacy by oral, in the aeromonosis and estreptococosis control in Nile tilapia. The ecotoxicological study will evaluate the fosfomicine environmental safety to aquaculture use. (AU)

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BARBUIO, Roberto. Eficácia terapêutica, segurança clínica e ecotoxicológico da fosfomicina em tilápias, Oreochromis niloticus, com aeromonose e estreptococose. 2017. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..

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