Sequencing and annotation of the genome from strain EPM 10906, a new mycobacterium species

• Grant number: 13/16018-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2013
• End date: August 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Sylvia Luisa Pincherle Cardoso Leão
• Grantee: Cristianne Kayoko Matsumoto
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 11/18326-4 - Mycobacteria and extrachromosomal elements: molecular characterization and biotechnological applications, AP.TEM

Abstract

The Mycobacterium chelonae-Mycobacterium abscessus group of rapidly-growing mycobacteria was recently revised, which resulted in the inclusion of new members (M. salmoniphilum e "M. franklinii"), and the reunion of three species species (M. abscessus, M. massiliense and M. bolletii) within a single species - M. abscessus, with the description of two M. abscessus subspecies (subsp. abscessus and subsp. bolletii). Our laboratory was responsible for some of these taxonomic updates. Now, we are studying a group of M. chelonae-M. abscessus isolates without a conclusive species identification. A subgroup includes two isolates from an outbreak in refractive surgery (LASIK) in São Paulo (1999) and one lymph node isolate from a patient from Ribeirão Preto (2007). These isolates showed the same PRA-hsp65 pattern of M. abscessus 1 (BstEII: 210, 235 bp; HaeIII: 145, 70, 60, 55 bp), except for the absence of a 70 bp HaeIII band. Sequence analysis of conserved genes revealed that these isolates were closely related to two zebrafish isolates from USA (JAN1 and JAN2), both without conclusive species identification. Phenotypic and molecular tests using these isolates will be carried out by a PhD student. The present project refers to the genome sequencing and annotation of one isolate (EPM 10906) from this particular subgroup. The objective is to complement the characterization of these isolates and to describe a new species from the M. chelonae-M. abscessus group.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA, CHRISTIANE LOURENCO; WHIPPS, CHRISTOPHER M.; MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; DROZ, SARA; TORTOLI, ENRICO; DE FREITAS, DENISE; CNOCKAERT, MARGO; PALOMINO, JUAN CARLOS; MARTIN, ANANDI; et al. Mycobacterium saopaulense sp nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 65, n. 12, p. 4403-4409, DEC 2015. (12/13763-0, 11/18326-4, 13/16018-6)

RIBEIRO, GIOVANNI MONTEIRO; MATSUMOTO, CRISTIANNE KAYOKO; REAL, FERNANDO; TEIXEIRA, DANIELA; DUARTE, RAFAEL SILVA; MORTARA, RENATO ARRUDA; LEAO, SYLVIA CARDOSO; CARVALHO-WODARZ, CRISTIANE DE SOUZA. Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp massiliense CRM0019 in alveolar epithelial cells. BMC Microbiology, v. 17, SEP 13 2017. (09/14665-9, 12/04913-8, 13/16018-6)

MACHADO, GABRIEL ESQUITINI; MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; DUARTE, RAFAEL DA SILVA; DE FREITAS, DENISE; PALACI, MOISES; HADAD, DAVID JAMIL; BATISTA LIMA, KARLA VALERIA; LOPES, MARIA LUIZA; RAMOS, JESUS PAIS; et al. Multilocus Sequence Typing Scheme versus Pulsed-Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates. Journal of Clinical Microbiology, v. 52, n. 8, p. 2881-2891, AUG 2014. (11/18326-4, 13/16018-6)

NASCIMENTO, HELOISA; VIANA-NIERO, CRISTINA; NOGUEIRA, CHRISTIANE LOURENCO; MARTINS BISPO, PAULO JOSE; PINTO, FERNANDO; PEREIRA UZAM, CAMILA DE PAULA; MATSUMOTO, CRISTIANNE KAYOKO; OLIVEIRA MACHADO, ANTONIA MARIA; LEAO, SYLVIA CARDOSO; HOFLING-LIMA, ANA LUISA; et al. Identification of the Infection Source of an Outbreak of Mycobacterium Chelonae Keratitis After Laser in Situ Keratomileusis. CORNEA, v. 37, n. 1, p. 116-122, JAN 2018. (12/13763-0, 13/16018-6, 11/18326-4)

NOGUEIRA, CHRISTIANE LOURENCO; WHIPPS, CHRISTOPHER M.; MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; DROZ, SARA; TORTOLI, ENRICO; DE FREITAS, DENISE; CNOCKAERT, MARGO; PALOMINO, JUAN CARLOS; MARTIN, ANANDI; et al. Mycobacterium saopaulense sp nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 65, p. 7-pg., 2015-12-01. (12/13763-0, 11/18326-4, 13/16018-6)