The ocular prosthesis is used for aesthetic rehabilitation of absent eye. The knowledge of its biocompatibility is important to ensure a safe use. The aim of this study is to evaluate the cytotoxicity of the materials used in the manufacture of ocular prosthesis, through the analysis of in vitro cell proliferation and the production of inflammatory mediators by conjunctiva cells. A total of 27 samples will be manufactured in the form of disc (10mmx1mm) and divided into 9 groups (n=3): N1 heat-polymerized resin by conventional method (RTC); N1 heat-polymerized resin by microwave energy (RTM); N1 auto-polymerized resin (RA); N1 resin + pigment, heat- polymerized by conventional method (RpTC); N1 resin + pigment, heat-polymerized by microwave energy (RpTM); N1 resin + pigment, auto-polymerized (RpA); Pigment polymerized by conventional method (pTC); Pigment polymerized by microwave energy (pTM); Pigment auto-polymerized (pA). For the preparation of extracts of these materials, samples will be placedin test tubes containing 9mL of DMEM and incubated at 37 oC for 24 hours. Then, the extracts citotoxicity will be evaluated by MTT assay in Chang conjunctiva cells culture. The production of cytokines IL-1, IL-6, TNF-± and chemokine CCL3/MIP-1± will be evaluated using ELISA and, the mRNA expression Colagen type I, TGF-² e MMP-9 by RT-PCR. Data will be submitted to the test of adherence to the normal curve, and appropriate statistical test will be applied and will be examined according to ISO-standard 10993-5, which describes the in vitro methods for the cytotoxicity evaluation.
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