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In vitro analysis of cell proliferation and inflammatory mediators production from conjunctival cells that are stimulated by ocular prostheses components

Grant number: 13/11830-4
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2013
Effective date (End): February 28, 2015
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal researcher:Marcelo Coelho Goiato
Grantee:Emily Vivianne Freitas da Silva
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The ocular prosthesis is used for aesthetic rehabilitation of absent eye. The knowledge of its biocompatibility is important to ensure a safe use. The aim of this study is to evaluate the cytotoxicity of the materials used in the manufacture of ocular prosthesis, through the analysis of in vitro cell proliferation and the production of inflammatory mediators by conjunctiva cells. A total of 27 samples will be manufactured in the form of disc (10mmx1mm) and divided into 9 groups (n=3): N1 heat-polymerized resin by conventional method (RTC); N1 heat-polymerized resin by microwave energy (RTM); N1 auto-polymerized resin (RA); N1 resin + pigment, heat- polymerized by conventional method (RpTC); N1 resin + pigment, heat-polymerized by microwave energy (RpTM); N1 resin + pigment, auto-polymerized (RpA); Pigment polymerized by conventional method (pTC); Pigment polymerized by microwave energy (pTM); Pigment auto-polymerized (pA). For the preparation of extracts of these materials, samples will be placedin test tubes containing 9mL of DMEM and incubated at 37 oC for 24 hours. Then, the extracts citotoxicity will be evaluated by MTT assay in Chang conjunctiva cells culture. The production of cytokines IL-1, IL-6, TNF-± and chemokine CCL3/MIP-1± will be evaluated using ELISA and, the mRNA expression Colagen type I, TGF-² e MMP-9 by RT-PCR. Data will be submitted to the test of adherence to the normal curve, and appropriate statistical test will be applied and will be examined according to ISO-standard 10993-5, which describes the in vitro methods for the cytotoxicity evaluation.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREITAS DA SILVA, EMILY VIVIANNE; GOIATO, MARCELO COELHO; BONATTO, LILIANE DA ROCHA; DE MEDEIROS, RODRIGO ANTONIO; DOS SANTOS, DANIELA MICHELINE; RANGEL, ELIDIANE CIPRIANO; PENHA DE OLIVEIRA, SANDRA HELENA. Toxicity analysis of ocular prosthesis acrylic resin with or without pigment incorporation in human conjunctival cell line. TOXICOLOGY IN VITRO, v. 36, p. 180-185, . (13/11830-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Emily Vivianne Freitas da. Análise in vitro da proliferação celular e produção de mediadores inflamatórios por células da conjuntiva estimuladas por materiais utilizados na confecção de próteses oculares. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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