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Analysis of DAMPs receptors (TLR4 and RAGE) in the process of alveolar bone repair in mice

Grant number: 13/08614-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2013
Effective date (End): August 31, 2014
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Carlos Eduardo Palanch Repeke
Grantee:Patrícia Sanches Kerges Bueno
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The search for an effective therapeutic for accelerating the bone healing is a challenge for researchers. Different methodologies and biomaterials are being applied, and the immune/inflammatory modulation is one of the most adopted strategies. If the exacerbated inflammatory response can delay or inhibit the bone formation, their presence in adequate intensity has become extremely important to the physiology of bone repair, demonstrating that the inflammatory response has a role complex role and it has been poorly understood. With the discovery of signaling mediated by receptors RAGE and TLR4, new possibilities was opened in the osteoimmunology field. These receptors play an important role in initiation of the immune/inflammatory response by recognizing damage associated molecular patterns (DAMPs), and they are able to produce several inflammatory mediators, such as pro-inflammatory cytokines and chemokines, which can influence in the bone repair. In this way, we aim to demonstrate the importance of TLR4 and RAGE, present in most of inflammatory cells in the bone repair process and its interaction with the inflammatory response triggered by DAMPs, produced during the upper incisor extraction surgery in mice. Furthemore, we will analyze the presence of TLR4 and RAGE in different stages of the alveolar repair and in different locations of the socket to be repaired. In addition, histological analysis of alveolar bone repair process will be performed. Thus, the results of this project will certainly help in a better understanding, not only of morphological events, but also the molecular events, aiming future therapeutic steps in order to interfere in positive way of the alveolar bone repair.