Influence of type 1 Diabetes Mellitus in the process of bone remodeling during rapid expansion of the jaw in rats

• Grant number: 13/10974-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2013
• End date: September 30, 2014
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Maria Bernadete Sasso Stuani
• Grantee: Fabio Santos Bottacin
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Diabetes mellitus is associated with several systemic changes and one of the most important complications of this disease is compromised bone healing. However, there is still no scientific information using molecular-level analysis of the influence of this disorder during the rapid expansion of the maxilla (ERM). Objective: The objective of this study is to evaluate the bone remodeling process during the implementation of ERM-induced diabetic rats. Material and Methods: One hundred and twenty male Wistar rats will be randomly divided into eight study groups, each containing 15 animals. Control groups: normal (N), with citrate buffer vehicle (V), type 1 diabetes mellitus induced by streptozotocin (D) type 1 diabetes induced with streptozotocin and treated with insulin (DT). Experimental groups: normal + ERM (Nd), type 1 diabetes induced with streptozotocin ERM + (Dd), type 1 diabetes induced with streptozotocin treated with insulin + associated ERM (DTD). The animals will be euthanized at 3, 7 and 10 days after RME. Analyzes will be conducted to evaluate the gene expression genes related to bone remodeling process: TNFRSF11A gene (RANK), Tnfsf11 (RANKL) and gene TNFRSF11B (OPG - osteoprotegerin). RT-real time PCR is performed to amplify the cDNA of the genes studied. The data will be tested for normality and if they have normal distribution to be analyzed using analysis of variance test two-way (two-way ANOVA) followed by Tukey post-test. The level of significance is 5%.