Study of the role of Toll-like receptors (TLRs) on CD200 induction in murine macrophages infected with Leishmania (L.) amazonensis

• Grant number: 13/14087-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2013
• Effective date (End): June 30, 2017
• Field of knowledge: Biological Sciences - Parasitology
• Principal Investigator: Mauro Javier Cortez Véliz
• Grantee: Ismael Pretto Sauter
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Parasites of the genus Leishmania are intracellular protozoan responsible to cause a complex of diseases called leishmaniosis. Once inside of the host, these parasites are phagocyted by defense immune cells, such as macrophages. Through the interaction of Toll-like receptors (TLRs), macrophages are activated and initiate the process of pathogen destruction. However, the parasites are capable to manipulate the defense responses of immune cells through the inhibition of the microbicidal mechanisms, leading to the survival and the chronicity of the infection. One of the immune modulatory mechanisms used by Leishmania to avoid macrophage responses is the expression of CD200, membrane glycoprotein that in contact with its receptor CD200R inhibits macrophage activation. Early studies in other pathogen models showed the link between TLRs and CD200, but in Leishmania model has not been investigated yet. In this context, the main goal of this project is to investigate if immunomodulation of CD200 molecule in macrophages induced by Leishmania is TLRs-dependent, as to understand the signaling pathways used in this process. For this, we propose to study the in vivo and in vitro infection events in different mice strains deficient in TLRs and verify the importance of these receptors on the expression of CD200 in Leishmania infection.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, THALITA C. S.; SAUTER, ISMAEL P.; BORDA-SAMPER, LINA; BENTIVOGLIO, ENYD; DAMATA, JARINA P.; TANIWAKI, NOEMI N.; ORREGO, PATRICIO R.; ARAYA, JORGE E.; LINCOPAN, NILTON; CORTEZ, MAURO. Effect of DODAB Nano-Sized Cationic Bilayer Fragments against Leishmania amazonensis. Molecules, v. 25, n. 23, DEC 2020. (13/14087-0, 13/15254-8, 16/08593-9, 12/24105-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SAUTER, Ismael Pretto. Role of Toll-like receptors (TLRs) in CD200 induction in murine macrophages infected with Leishmania (Leishmania) amazonensis.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.