Chemical Study of EtOAc fraction from Vochysia divergens and in vitro schistossomicidal potential evaluation

• Grant number: 13/15774-1
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2013
• Effective date (End): September 30, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal researcher: Ana Helena Januário
• Grantee: Luis Claudio Kellner Filho
• Home Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

This project intends to realize the chemistry study of leaves EtOAc fraction obtained from the leaves ethanolic crude extract of Vochysia divergens Pohl (Vochysiaceae), a native species from Pantanal Biome. This region has been selected for study within the Regular Research Project "Bioactive compounds from medicinal and food plants of the Cerrado and Pantanal and their endophytic microbiota (Fapesp Process # 2011/00631-5). The selected fraction showed activity in in vitro schistosomicidal assay carried out by our research group. Therefore, the the phytochemistry approach will be bioassay-guided on this activity. The proposed schistosomicidal test will be held evaluating the life cycle of Schistosoma mansoni vector. The analytical methodologies will employ chromatography techniques (CC and HPLC-DAD). The chemical structures of the isolated compounds will the assign on the bases of 1D and 2D NMR experiments. Thus, the main goal of this proposal will be identify the potential schistossomicidal of natural compounds, as well as contribute to the human resource formation in the natural product area in Brazil. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIMENTAA, LETICIA PEREIRA; KELLNER FILHO, LUIS CLADIO; SOARES PARPINELLI, BRUNA ARRUDA; DE SIQUEIRA, KATIA APARECIDA; SOARES, MARCOS ANTONIO; ANDRADE E SILVA, MARCIO LUIS; CUNHA, WILSON ROBERTO; PAULETTI, PATRICIA MENDONCA; JANUARIO, ANA HELENA. DEVELOPMENT AND VALIDATION OF AN HPLC-DAD ANALYTICAL METHOD TO QUANTIFY 5-METHOXYFLAVONES IN METHANOLIC EXTRACTS OF Vochysia divergens POHL CULTURED UNDER STRESS CONDITIONS. Química Nova, v. 40, n. 9, p. 971-975, SEP 2017. (13/15774-1, 11/00631-5, 13/22030-9, 16/08057-0)