|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||October 01, 2013|
|Effective date (End):||August 31, 2015|
|Field of knowledge:||Biological Sciences - Botany - Applied Botany|
|Principal researcher:||Alexandra Christine Helena Frankland Sawaya|
|Grantee:||Claudia de Lazzari Almeida|
|Home Institution:||Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil|
The use of medicinal plants and phytotherapic medicine is being increasingly stimulated by government policies at all levels. Syrup based on Mikania glomerata Spreng.is presently furnished by the Brazilian Health System (SUS) as medicine for cough and bronchitis. However, variations in the cultivation conditions of medicinal plants may result in different concentrations of active components, affecting the safety, quality and efficiency expected of herbal medicines. Few studies regarding the cultivation of the two species of interest as medicinal plants, Mikania glomerata e Mikania laevigata Schultz, were found. To this moment, the fluctuations in the concentrations of secondary metabolites in these species due to environmental variations have not been satisfactorily evaluated. As both species are found in the 1st Brazilian Phytotherapic Formulary, and apparently may be used indiscriminately, they shall be studied in parallel. We will evaluate how the secondary metabolites are influenced by variations in the following cultivation conditions: temperature, luminosity, soil fertility and pH; and mechanical damage. After the treatments, the extracts of the leaves will be evaluated by liquid chromatography-mass spectrometry, following the differences in the concentration of their chemical marker, coumarin, and the profile of the other components, as well as the levels of chlorophyll, starch and sugars. These results will allow us to determine the best conditions for these two species to be grown in order to minimize the variations in contents of active substances; and may be used as a standard condition for the cultivation these medicinal plants.