EVALUATION OF PHOSPHORYLATION OF AKT AND CONTENT OF GLUCOSE TRANSPORTER PROTEIN GLUT4 IN SKELETAL MUSCLE OF RATS WITH PERIAPICAL LESION

• Grant number: 13/14924-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): November 01, 2013
• Effective date (End): December 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Doris Hissako Matsushita
• Grantee: Renato Felipe Pereira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Currently, there is some consensus within the dental area related to the fact that chronic inflammation in teeth may eventually lead to systemic disorders. Periapical lesion (PL) is characterized as an oral inflammation and it is associated to the increase of proinflammatory citokines, such as IL-6 and TNF-alpha, that can possibly induce insulin resistance. Insulin resistance can be defined as the state in which there is a glucose uptake decrease by tissue in response to insulin stimulation, however, the mechanisms that cause insulin resistance are not totaly understood. Former studies from our laboratory demonstrated that adult rats with periapical lesion showed changes in the initial stage of insulin signaling and lower insulin sensitivity. Knowing this, more studies are needed to verify whether these changes are also present in the continuity of the insulin signaling cascade. The objectives of this study will be to evaluate in rats with PL: 1) plasma glucose and insulin levels, 2) status of serine/ threonine phosphorylation of Akt in skeletal muscle gastrocnemius (GM), 3) content of protein GLUT4 and its translocation index for plasma membrane in GM. Therefore, it will be used 30 Wistar rats (2 months old) divided into two groups: a) control rats without PL b) mice with PL induced in right upper molars by using a carbon steel drill fitted with ball 0.1 mm. Statistical analysis will be performed by the method of Student's t test for unpaired samples, and the differences between the two groups will be considered significant when p <0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, RENATO FELIPE; DE OLIVEIRA DA MOTA, MAX SANDER; DE LIMA COUTINHO MATTERA, MARIA SARA; SAORI TSOSURA, THAIS VERONICA; CHIBA, FERNANDO YAMAMOTO; SALIBA GARBIN, CLEA ADAS; ERVOLINO, EDILSON; ANGELO CINTRA, LUCIANO TAVARES; OKAMOTO, MARISTELA MITIKO; MACHADO, UBIRATAN FABRES; et al. Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle. CLINICAL ORAL INVESTIGATIONS, v. 20, n. 7, p. 1625-1630, SEP 2016. (13/14924-0)