IMPACT OF INCIDENT INFECTION BY GBV-C IN CELL ACTIVATION IN PEOPLE LIVING WITH HUMAN IMMUNODEFICIENCY VIRUS (HIV).

• Grant number: 13/05246-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): December 01, 2013
• Effective date (End): October 31, 2016
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Esper Georges Kallás
• Grantee: Dayane Alves Costa
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The GBV-C, also called hepatitis G virus (HGV) is a RNA virus of the Flaviviridae family and has similar genome to the hepatitis C virus (HCV). No diseases were related to the presence of GBV-C and recent studies have shown positive association between GBV-C and HIV viruses. Previous studies of our group revealed the importance to understand this co-infection. Therefore, the aim of this study is to evaluate the relationship between incident infection by GBV-C and decreased expression of activation markers in people living with HIV. Samples from patiens of the Laboratory of Medical Investigation 60, Department of Clinical Immunology and Allergy, University of São Paulo will be selected for the study. Patients included in the study will be newly infected with HIV. To test recent HIV seroconversion in these patients the algorithm seroconversion test for recent HIV seroconversion (STARHS) will be applied and those with ELISA HIV positive test will be excluded from the study . The counting of CD4 + and CD8 + will be by labeling with monoclonal antibodies to molecules CD3, CD4, CD8 and CD45 conjugated with fluorochromes. Added to this, the profile of cell activation will be obtained by staining lymphocytes with antibodies to CD3, CD4, CD8, CD38, HLA-DR and CCR5. For this purpose cryopreserved peripheral blood mononuclear cells (PBMC) derived from individuals infected with HIV-1 will be used and the presence of GBV-C will be determined using RNA tests and the viral load of HIV and GBV-C will be analyzed by commercial kits. Finally, the presence of CCR5*”32 deletion in the co-infected people will be evaluated, since its presence may be an indicative of immune responses positive regulation in recently infected HIV patients. Therefore, high prevalence of GBV-C in these patients shows a relationship of positive viral interaction, and has a negative impact in the progression of HIV infection.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AVELINO-SILVA, VIVIAN I.; MIYAJI, KARINA T.; HUNT, PETER W.; HUANG, YONG; SIMOES, MARISOL; LIMA, SHEILA B.; FREIRE, MARCOS S.; CAIAFFA-FILHO, HELIO H.; HONG, MARISA A.; COSTA, DAYANE ALVES; et al. CD4/CD8 Ratio and KT Ratio Predict Yellow Fever Vaccine Immunogenicity in HIV-Infected Patients. PLoS Neglected Tropical Diseases, v. 10, n. 12, DEC 2016. (13/05246-8)

AVELINO-SILVA, VIVIAN IIDA; MIYAJI, KARINA TAKESAKI; MATHIAS, AUGUSTO; COSTA, DAYANE ALVES; DE CARVALHO DIAS, JULIANA ZANATTA; LIMA, SHEILA BARBOSA; SIMOES, MARISOL; FREIRE, MARCOS S.; CAIAFFA-FILHO, HELIO H.; HONG, MARISA A.; et al. CD4/CD8 Ratio Predicts Yellow Fever Vaccine-Induced Antibody Titers in Virologically Suppressed HIV-Infected Patients. JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, v. 71, n. 2, p. 189-195, FEB 1 2016. (13/05246-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

COSTA, Dayane Alves. The impact of GBV-C incident infection on cell activation in human immunodeficiency virus (HIV)-infected patients. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.