Among the most useful natural products, polyketides and non-ribosomal peptides share high molecular complexity and therapeutic activity. The production of these metabolites has been observed in many organisms, however, the cultivable actinobacteria are certainly the most prolific and versatile producers of such metabolites. In the case of actinobacteria, the assembly-line enzimology of modular polyketide synthetases (PKSs) and nonribosomal peptides synthetases (NRPSs) are finely established. With the fast advances in sequencing methodologies and availability of bioinformatics tools it is possible to map the presence of biosynthetic genes that encode for these and other classes of enzymes and multi enzymatic complexes in order to estimate the potential in biosynthesize secondary metabolites. The versatility of this tool, known as genome mining allows the search for new therapeutic targets.In the present study we intend to construct and screen a genomic PAC library from an endophytic strain, Streptomyces sp, in order to characterize the gene cluster involved in the biosynthesis of a putative metabolite originated from interative action of type 1 PKS, NRPS and trans AT PKS. The cluster will be transferred to a heterologous host (S. coelicolor and S. lividans) and heterologous production will be to be evaluated.
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