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Mining a of t1PKS-transAT PKS-NRPS in Streptomyces sp.

Grant number: 13/12598-8
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2013
Effective date (End): July 31, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal researcher:Luciana Gonzaga de Oliveira
Grantee:Renata Sigrist
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Among the most useful natural products, polyketides and non-ribosomal peptides share high molecular complexity and therapeutic activity. The production of these metabolites has been observed in many organisms, however, the cultivable actinobacteria are certainly the most prolific and versatile producers of such metabolites. In the case of actinobacteria, the assembly-line enzimology of modular polyketide synthetases (PKSs) and nonribosomal peptides synthetases (NRPSs) are finely established. With the fast advances in sequencing methodologies and availability of bioinformatics tools it is possible to map the presence of biosynthetic genes that encode for these and other classes of enzymes and multi enzymatic complexes in order to estimate the potential in biosynthesize secondary metabolites. The versatility of this tool, known as genome mining allows the search for new therapeutic targets.In the present study we intend to construct and screen a genomic PAC library from an endophytic strain, Streptomyces sp, in order to characterize the gene cluster involved in the biosynthesis of a putative metabolite originated from interative action of type 1 PKS, NRPS and trans AT PKS. The cluster will be transferred to a heterologous host (S. coelicolor and S. lividans) and heterologous production will be to be evaluated.

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SIGRIST, RENATA; PAULO, BRUNO S.; ANGOLINI, CELIO F. F.; DE OLIVEIRA, LUCIANA G. Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 157 MAR 2020. Web of Science Citations: 0.
PAULO, BRUNO S.; SIGRIST, RENATA; ANGOLINI, CELIO F. F.; DE OLIVEIRA, LUCIANA G. New Cyclodepsipeptide Derivatives Revealed by Genome Mining and Molecular Networking. CHEMISTRYSELECT, v. 4, n. 27, p. 7785-7790, JUL 23 2019. Web of Science Citations: 1.
BRUNO S. PAULO; RENATA SIGRIST; CÉLIO F. F. ANGOLINI; MARCOS N. EBERLIN; LUCIANA G. DE OLIVEIRA. Gene Deletion Leads to Improved Valinomycin Production by Streptomyces sp. CBMAI 2042. Journal of the Brazilian Chemical Society, v. 30, n. 3, p. 673-679, Mar. 2019.
PAULO, BRUNO S.; SIGRIST, RENATA; ANGOLINI, CELIO F. F.; EBERLIN, MARCOS N.; DE OLIVEIRA, LUCIANA G. Gene Deletion Leads to Improved Valinomycin Production by Streptomyces sp. CBMAI 2042. Journal of the Brazilian Chemical Society, v. 30, n. 3, SI, p. 673-679, MAR 2019. Web of Science Citations: 2.
BRUNO S. PAULO; RENATA SIGRIST; LUCIANA G. DE OLIVEIRA. AVANÇOS RECENTES EM BIOSSÍNTESE COMBINATÓRIA DE POLICETÍDEOS: PERSPECTIVAS E DESAFIOS. Química Nova, v. 42, n. 1, p. 71-83, Jan. 2019.
DE OLIVEIRA, LUCIANA GONZAGA; SIGRIST, RENATA; PAULO, BRUNO SACHETTO; SAMBORSKYY, MARKYIAN. Whole-Genome Sequence of the Endophytic Streptomyces sp. Strain CBMAI 2042, Isolated from Citrus sinensis. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 8, n. 2 JAN 2019. Web of Science Citations: 1.
CÉLIO F. F. ANGOLINI; ANA B. GONÇALVES; RENATA SIGRIST; BRUNO S. PAULO; MARKIYAN SAMBORSKYY; PEDRO L. R. CRUZ; ADRIANA F. VIVIAN; EDUARDO M. SCHMIDT; MARCOS N. EBERLIN; WELINGTON L. ARAÚJO; LUCIANA G. DE OLIVEIRA. Genome Mining of Endophytic Streptomyces wadayamensis Reveals High Antibiotic Production Capability. Journal of the Brazilian Chemical Society, v. 27, n. 8, p. 1465-1475, Ago. 2016.
SIGRIST, RENATA; DA COSTA, BRUNA ZUCOLOTO; MARSAIOLI, ANITA JOCELYNE; DE OLIVEIRA, LUCIANA GONZAGA. Nature-inspired enzymatic cascades to build valuable compounds. BIOTECHNOLOGY ADVANCES, v. 33, n. 5, SI, p. 394-411, SEP-OCT 2015. Web of Science Citations: 18.

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