Use of NPPC, folicular fluid and BMP15 to modulate oocyte meiotic arrest on in vitro maturation: effects on cumulus-oocyte complexes and bovine embryo production

• Grant number: 13/07730-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2013
• End date: March 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcelo Fábio Gouveia Nogueira
• Grantee: Elisa Mariano Pioltine
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated research grant: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle, AP.TEM

Abstract

Studies in mice demonstrated that C-type natriuretic peptide precursor (NPPC) and its receptor type 2 (NPR2) are essential for the maintenance of meiotic arrest in the oocyte. Paracrine factors secreted by the oocyte (such as bone morphogenetic protein or BMP15), seem to modulate the system stimulating NPR2 gene expression in cumulus cells. Consequently, the NPPC, NPR2 and BMP15 system arises as a critical mechanism controlling oocyte meiosis resumption in vitro, potentially applicable in the bovine species. Recently, in bovine model, oocyte nuclear maturation was delayed when maturation media was supplemented with bovine follicular fluid (bFF) and, consequently, embryo production in vitro was improved. However, the causative factors present in the bFF responsible for the delay are not known. Estradiol (a component of bFF) directly stimulates NPR2 in the cumulus cells. Therefore, the objective of this project is to characterize bovine oocyte meiosis in vitro through modulation of NPPC NPR2, BMP15, bFF and estradiol. Specifically, we aim to: 1) detect NPPC and its receptor NPR2 in bovine ovarian follicle, 2) assess the effects of bFF, estradiol and NPPC on nuclear maturation and in vitro production of embryos, and 3) evaluate the effects of BMP15 on the oocyte meiotic arrest. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIOLTINE, ELISA M.; MACHADO, MARIANA F.; DA SILVEIRA, JULIANO C.; FONTES, PATRICIA K.; BOTIGELLI, RAMON C.; QUAGLIO, V, ANA ELISA; COSTA, CAMILA B.; NOGUEIRA, MARCELO F. G.. Can extracellular vesicles from bovine ovarian follicular fluid modulate the in-vitro oocyte meiosis progression similarly to the CNP-NPR2 system?. Theriogenology, v. 157, p. 210-217, NOV 2020. (12/50533-2, 13/07730-4)

BOTIGELLI, RAMON CESAR; RAZZA, EDUARDO MONTANARI; PIOLTINE, ELISA MARIANO; FONTES, PATRICIA KUBO; LANCELLOTTI SCHWARZ, KATIA REGINA; VERDE LEAL, CLAUDIA LIMA; GOUVEIA NOGUEIRA, MARCELO FABIO. Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos. REPRODUCTIVE BIOLOGY, v. 18, n. 1, p. 66-75, MAR 2018. (12/23409-9, 15/20379-0, 13/05083-1, 12/50533-2, 13/07730-4, 12/00170-0)

BOTIGELLI, RAMON CESAR; RAZZA, EDUARDO MONTANARI; PIOLTINE, ELISA MARIANO; GOUVEIA NOGUEIRA, MARCELO FABIO. New approaches regarding the in vitro maturation of oocytes: manipulating cyclic nucleotides and their partners in crime. JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA, v. 21, n. 1, p. 35-44, JAN-MAR 2017. (12/23409-9, 13/05083-1, 12/50533-2, 13/07730-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PIOLTINE, Elisa Mariano. Sistema NPPC-NPR2 na espécie bovina: identificação em folículos antrais e modulação na concentração de nucleotídeos cíclicos e na expressão gênica de PDE3 em complexos cumulus-oócito. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências e Letras. Assis Assis.